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Anti myc tag antibody

Manufactured by Proteintech
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The Anti-Myc-tag antibody is a laboratory reagent used to detect proteins tagged with the Myc epitope. It is a highly specific antibody that recognizes the Myc-tag sequence, a commonly used protein tag for various applications such as protein expression and purification. The antibody can be used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to identify and study Myc-tagged proteins.

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Lab products found in correlation

Anti β actin antibody, by Proteintech (2 mentions) Rat anti mouse cd68 monoclonal antibody, by Bio-Rad (1 mentions) Revertra ace qpcr rt master mix, by Toyobo (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647 goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions) Anti total akt antibody, by Cell Signaling Technology (1 mentions) Faststart universal sybr green master mix, by Roche (1 mentions) Anti gapdh, by Abcam (1 mentions) Rabbit anti iba1, by Fujifilm (1 mentions) Alexa fluor 594 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Anti phospho akt ser473, by Cell Signaling Technology (1 mentions) Anti ha tag, by Proteintech (1 mentions) β catenin 6b3 rabbit mab, by Cell Signaling Technology (1 mentions) Anti csf1r, by Abcam (1 mentions) Dead end fluorometric tunnel system, by Promega (1 mentions) Basic glial cells nucleofector kit, by Lonza (1 mentions) Brdu cell proliferation elisa kit, by Abcam (1 mentions) Csf 1, by R&D Systems (1 mentions) Granulocyte macrophage colony stimulating factor gm csf, by R&D Systems (1 mentions) Anti flag monoclonal antibody, by Merck Group (1 mentions)

Spelling variants of this product

Anti-Myc (39 citations) Myc-tag (16 citations) Anti-MYC-tag (8 citations) Myc-tag antibody (5 citations) Anti-myc antibody (4 citations) Anti-TM (2 citations)

4 protocols using anti myc tag antibody

1

Antibody-based Signaling Pathway Analysis

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Phospho-CSF1R (Tyr723) (49C10) Rabbit mAb (RRID: AB_2085229), β-catenin (6B3) Rabbit mAb (RRID: AB_331149), anti-Phospho-Akt (Ser473) (RRID: AB_329825) and anti-total-Akt antibodies (RRID: AB_329827) were all bought from Cell Signaling Technology. Anti-GAPDH (RRID: AB_2630358), anti-CSF1R (RRID: AB_2085251), anti-Aβ (MOAB2), anti-β-actin antibodies (RRID: AB_306371) and Alexa Fluor 647 Donkey Anti-Rat IgG H&L (RRID: AB_2813835), as well as the BrdU Cell proliferation ELISA Kit, were all from Abcam. Rat Anti-mouse CD68 monoclonal antibody (RRID: AB_322219) was from Bio-Rad. Anti-HA-tag (RRID: AB_11042321) antibody and anti-Myc-tag antibody (RRID: AB_11182162) were from Proteintech. Rabbit anti-Iba1 (RRID: AB_2687911) was from Wako. Alexa Fluor 488 Goat anti-Rabbit (RRID: AB_10374301), Alexa Fluor 594 Goat anti-Rabbit (RRID: AB_10374440), Alexa Fluor 647 Goat anti-Rabbit antibodies (RRIDAB_10371940) and TRIzol reagent were all from Thermo Fisher Scientific. Granulocyte-macrophage colony stimulating factor (GM-CSF) and CSF1 were from R&D Systems. Basic Glial Cells Nucleofector Kit was purchased from LONZA. ReverTra Ace qPCR RT Master Mix was from TOYOBO. FastStart Universal SYBR Green Master mix was from Roche. CellTiter 96R Aqueous One Solution (MTS assay) and DeadEnd™ Fluorometric TUNNEL System were from Promega.
Cheng B., Li X., Dai K., Duan S., Rong Z., Chen Y., Lü L., Liu Z., Huang X., Xu H., Zhang Y.W, & Zheng H. (2021). Triggering Receptor Expressed on Myeloid Cells-2 (TREM2) Interacts With Colony-Stimulating Factor 1 Receptor (CSF1R) but Is Not Necessary for CSF1/CSF1R-Mediated Microglial Survival. Frontiers in Immunology, 12, 633796.
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2

Antibody Detection in Whole-Cell Lysates

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Antibodies were obtained from the following companies: monoclonal anti-FLAG antibody (F1804, Sigma-Aldrich), anti-MYC-Tag antibody (66004-I-Ig, Proteintech, Chicago, IL, USA), and anti-Nectin-2 antibody (ab135246, Abcam, Cambridge, UK). Whole-cell lysates were prepared using RIPA lysis buffer (Millipore), with complete protease inhibitors (Roche, Basel, Switzerland). The BCA Protein Assay Kit (Thermo Fisher) was used to determine the protein concentration. The HRP-conjugated anti-rabbit IgG (1:10000, Sigma-Aldrich) or anti-mouse IgG (1:10000, Sigma-Aldrich) were used to reveal antibody binding. Immunoreactive complexes were detected using Immobilon Western Chemiluminescent HRP Substrate (Millipore) and exposed to a GE Amersham Imager 600 machine.
Wang C., Li Q., Lv J., Sun X., Cao Y., Yu K., Miao C., Zhang Z.S., Yao Z, & Wang Q. (2019). Alpha-hemolysin of uropathogenic Escherichia coli induces GM-CSF-mediated acute kidney injury. Mucosal Immunology, 13(1), 22-33.
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3

Antibody Characterization for SRBSDV P10

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The anti-SRBSDV P10 antibody was kindly gifted by Professor Jianxiang Wu (Zhejiang University). The other antibodies were purchased: anti-β actin antibody (Proteintech, 66008), anti-P-JNK antibody (Bioss, BS-4163R), anti-Atg8 antibody (Abgent, AP1802a), anti-LAMP1 antibody (Bioss, BSM-51301M), goat anti-mouse IgG+HRP antibody (Solarbio, SE131), anti-Myc tag antibody (Proteintech, 66004), anti-His tag antibody (Cell Signaling Technology, 2365), Dylight 488 goat anti-rabbit IgG (Abbkine, A23220), Dylight 488 goat anti-mouse IgG (Abbkine, A23210), Dylight 549 goat-anti-mouse IgG (Abbkine, A23310), goat anti-mouse IgG+HRP (Thermo Fisher, 32430), goat anti-rabbit IgG+HRP (Thermo Fisher, 32460). Other reagents used and their sources are mounting medium with 4′6-diamidino-2-phenylindole (DAPI) (Abcam, AB104139), rapamycin (Solarbio, IR0010), 3-methyladenine (3-MA) (Solarbio, IM0190), anisomycin (TargetMol, T6758), SP600125 (Abcam, AB120065).
Zhang L., Liu W., Wu N., Wang H., Zhang Z., Liu Y, & Wang X. (2023). Southern rice black-streaked dwarf virus induces incomplete autophagy for persistence in gut epithelial cells of its vector insect. PLOS Pathogens, 19(1), e1011134.
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4

Antibody and Reagent Identification for Cell Signaling

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Primary antibodies used in this study included Anti-IL-3Rα antibody (ABclonal, A3926), anti-STAT5 antibody (Cell Signaling Technology, 94205), anti-phospho-STAT5 antibody (Cell Signaling Technology, 4322), anti-β-actin antibody (Proteintech Group, 66009-1-Ig), anti-RNF128 antibody (Cell Signaling Technology, 71,590), anti-LC3B antibody (Cell Signaling Technology, 3868), anti-Flag-tag antibody (Sigma-Aldrich, F1804), anti-HA-tag antibody (Invitrogen, 26,183), and anti-Myc-tag antibody (Proteintech Group, 16286-1-AP) were employed. Secondary antibodies included HRP conjugated goat anti-rabbit IgG (Boster Bio, BA1054), HRP conjugated goat anti-mouse IgG (Boster Bio, BA1050), TRITC conjugated goat anti-mouse IgG (Boster Bio, BA1089), DyLight®488 conjugated goat anti-mouse IgG (Boster Bio, BA1126), TRITC conjugated goat anti-rabbit IgG (Boster Bio, BA1090), and DyLight®488 conjugated goat anti-rabbit IgG–488 (Boster Bio, BA1127). All antibodies were purchased from the respective suppliers as indicated. Recombinant human IL-3 (Pepro Tech, 200-03), mouse Il-3 (MCE, HY-P7062), mouse GM-CSF (Pepro Tech, 213–13) and LPS (Sigma-Aldrich, L2880) were purchased from the respective suppliers. MG132 (HY-13259), Chloroquine (HY-17589 A), and Cycloheximide (HY-12320) were obtained from MCE (MedChemExpress).
Yu J., Li J., Shen A., Liu Z, & He T.S. (2024). E3 ubiquitin ligase RNF128 negatively regulates the IL-3/STAT5 signaling pathway by facilitating K27-linked polyubiquitination of IL-3Rα. Cell Communication and Signaling : CCS, 22, 254.
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