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Cd45ra apc efluor780 hi100

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CD45RA-APC-eFluor780 (HI100) is a fluorescently-labeled antibody that can be used for the detection and analysis of the CD45RA surface marker on cells. It is designed for use in flow cytometry applications.

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Lab products found in correlation

Lsr 2 cytometer, by BD (3 mentions) Cd19 percp cy5, by BioLegend (3 mentions) Cd8 percp cy5, by BioLegend (3 mentions) Cd3 pacific blue, by BD (3 mentions) Cd4 alexafluor700, by BD (2 mentions) Cd66b percp cy5.5 g10f5, by BioLegend (2 mentions) Ccr7 fitc 150503, by R&D Systems (2 mentions) Hiv p24 rd1 fh190 1 1, by Beckman Coulter (2 mentions) Cd3 alexa700, by BioLegend (2 mentions) Hla dr bv785, by BioLegend (2 mentions) Cd326 bv650, by BioLegend (2 mentions) Cd4 fitc sfci12t4d11, by Beckman Coulter (2 mentions) Cd4 fitc, by Beckman Coulter (1 mentions) Ccr4 pe cy7 1g1, by BD (1 mentions) Cd3 alexa700, by Thermo Fisher Scientific (1 mentions) Cd4 alexa fluor 700 rpa t4, by BD (1 mentions) Cd66b percp cy5, by BioLegend (1 mentions) Liberase dl, by Roche (1 mentions)

Close spelling variants of this product

CD45RA APC (8 citations) CD45RA (HI100, (2 citations)

3 protocols using cd45ra apc efluor780 hi100

1

Polychromatic Flow Cytometry Analysis

Cited 1 time
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The following fluorochrome-conjugated Abs were used for polychromatic flow cytometry analysis: CD3-Pacific-blue (UCHT1), CD4-Alexa-Fluor 700 (RPA-T4), CCR4-PE-Cy7 (1G1), CCR6-PE (11A9), CCR7-PE-Cy7 (3D12), CXCR3-PE-Cy5 (1C6) (BD Biosciences, San Diego, CA), CCR7-FITC (150503) (R&D Systems, Minneapolis, MN), CD45RA-APC-eFluor780 (HI100) (eBioscience, San Diego, CA), CD3-Alexa700 (UCHT1), CD326-BV650 (9C4), CD8-PerCP-Cy5.5 (RPA-T8), CD19-PerCP-Cy5.5 (HIB19), CD66b-PerCP-Cy5.5 (G10F5), HLADR-BV785 (L243; BioLegend), CD4-FITC (SFCI12T4D11) and HIV-p24-RD1 (FH190-1-1; Beckman Coulter, Fullerton, CA). Live/Dead Fixable Aqua Dead Cell Stain Kit (Vivid; Life Technologies, Burlington, ON) was used to exclude dead cells. Cells were analyzed by FACS using the BD-LSRII cytometer and BD-Diva (BD Biosciences, San Jose, CA), and FlowJo (©Tree Star, Inc., Ashland, OR) softwares. All Abs were titrated for an optimal noise/signal ratio and Ab cocktails were validated by comparing single to multiple staining. Positive gates were placed based on fluorescence minus one (FMO) controls [41 (link), 69 ]
Gosselin A., Wiche Salinas T.R., Planas D., Wacleche V.S., Zhang Y., Fromentin R., Chomont N., Cohen É.A., Shacklett B., Mehraj V., Ghali M.P., Routy J.P, & Ancuta P. (2016). HIV persists in CCR6+CD4+ T cells from colon and blood during antiretroviral therapy. AIDS (London, England), 31(1), 35-48.
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2

Polychromatic Flow Cytometry of Immune Markers

Cited 1 time
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The following fluorochrome-conjugated antibodies (Abs) were used for polychromatic flow cytometry analysis: CD3-Pacific-blue (UCHT1), CD4-Alexa-Fluor 700 (RPA-T4), CCR4-PE-Cy7 (1G1), CCR6-PE (11A9), CCR7-PE-Cy7 (3D12), CXCR3-PE-Cy5 (1C6) (BD Biosciences, San Diego, California, USA), CCR7-FITC (150503) (R&D Systems, Minneapolis, Minnesota, USA), CD45RA-APC-eFluor780 (HI100) (eBioscience, San Diego, California, USA), CD3-Alexa700 (UCHT1), CD326-BV650 (9C4), CD8-PerCP-Cy5.5 (RPA-T8), CD19-PerCP-Cy5.5 (HIB19), CD66b-PerCP-Cy5.5 (G10F5), human leukocyte antigen - antigen D related (HLA-DR)-BV785 (L243; Biolegend ,San Diego, California USA), CD4-FITC (SFCI12T4D11), and HIV-p24-RD1 (FH190–1–1); (Beckman Coulter, Fullerton, California, USA). Live/Dead Fixable Aqua Dead Cell Stain Kit (Vivid; Life Technologies, Burlington, Ontario, Canada) was used to exclude dead cells. Cells were analyzed by fluorescence-activated cell sorting (FACS) using the BD-LSRII cytometer and BD-Diva (BD Biosciences) and FlowJo (Tree Star, Inc., Ashland, Oregon, USA) softwares. All Abs were titrated for an optimal noise/signal ratio and Ab cocktails were validated by comparing single with multiple staining. Positive gates were placed based on fluorescence minus one controls [41 (link),69 ].
Gosselin A., Wiche Salinas T.R., Planas D., Wacleche V.S., Zhang Y., Fromentin R., Chomont N., Cohen É.A., Shacklett B., Mehraj V., Ghali M.P., Routy J.P, & Ancuta P. (2016). HIV persists in CCR6+CD4+ T cells from colon and blood during antiretroviral therapy. AIDS (London, England), 31(1), 35-48.
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3

Immune Cell Profiling in Sigmoid Colon

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Sigmoid colon biopsies (SCB) were processed using Liberase DL (Roche Diagnostics). Matched blood was collected and PBMCs)were isolated by gradient density centrifugation. Cells were stained with the following fluorochrome-conjugated antibodies: CD3-Pacific-Blue (UCHT1), CD4-Alexa-Fluor 700 (RPA-T4; BD Biosciences), CD45RA-APC-eFluor780 (HI100) (eBioscience), CD3-Alexa700 (UCHT1), CD326-BV650 (9C4), CD8-PerCP-Cy5.5 (RPA-T8), CD19-PerCP-Cy5.5 (HIB19), CD66b-PerCP-Cy5.5 (G10F5; Biolegend), CD4-FITC (SFCI12T4D11; Beckman Coulter), CD64-APC (10.1.1; Miltenyi). Aqua Vivid Dead Cell Stain Kit (Life Technologies) was used to exclude dead cells. Phenotypic analysis was performed by flow cytometry using BD-LSRII cytometer, BD-Diva (BD Biosciences) and FlowJo software (Tree Star). Detailed sample processing informations are included in our previous publication [70 (link)].
Gabriel E.M., Wiche Salinas T.R., Gosselin A., Larouche-Anctil E., Durand M., Landay A.L., El-Far M., Tremblay C.L., Routy J.P, & Ancuta P. (2021). Overt IL-32 Isoform Expression at Intestinal Level during HIV-1 Infection is Negatively Regulated by IL-17A. AIDS (London, England), 35(12), 1881-1894.
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