Glycidol

Glycidol (purity 96.0%) and fluorescein isothiocyanate (FITC; purity ≥ 90.0%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Glycidyl oleate was purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). L-d₈-valine (purity 98.0%), which was used for the synthesis of the Glycidol internal standard 3-(fluorescein-5-yl)-1-(2,3-dihydroxypropyl)-5-d₇-isopropyl-2-thioxo-4-imidazolidinone (DHP-Val-d₇-FTH) [12 (link)], was obtained from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). Lipase Kit S was obtained from DS Pharma Biomedical Co., Ltd. (Osaka, Japan). All other chemicals and solvents used were of analytical grade.

FITC (purity ≥ 90.0%), glycidol (purity 96.0%), human hemoglobin lyophilized powder (Product Number H7379), and human serum albumin lyophilized powder (purity ≥ 96.0%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Acrylamide (purity 95.0%) was obtained from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan). L-Valine-(¹³C₅) (purity 98.0%), used for the synthesis of the glycidol internal standard N-(2,3-dihydroxypropyl)-(¹³C₅)-valine, and L-d8-valine (purity 98.0%), used for the synthesis of the Acrylamide internal standard fluorescein-5-[4-d7-isopropyl-3-(2-carbamoylethyl)-2-thioxo-imidazolidin5-one] (AA-d7-Val-FTH), was obtained from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). All other chemicals and solvents used were of analytical grade.