Cd16 32 antibody fc block 2.4g2

Manufactured by BD

The CD16/32 antibody (Fc block; 2.4G2) is a laboratory reagent used to block Fc receptors on cells. It is commonly used in flow cytometry and other immunological assays to prevent non-specific binding of antibodies to Fc receptors, thereby improving the specificity of the assay.

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Lab products found in correlation

Fortessa, by BD (2 mentions) Cd86 apc, by BioLegend (1 mentions) Cd25 apc cy7, by BioLegend (1 mentions) Cd11c apc cy7, by BioLegend (1 mentions) Cytofix cytoperm, by BD (1 mentions) Monensin and brefeldin a, by BD (1 mentions) Perm wash buffer, by BD (1 mentions)

Spelling variants of this product

Fc block (765 citations) CD16/32 (49 citations) Fc block (2.4G2, (41 citations) CD16/32 (2.4G2) (22 citations) CD16/32 (Fc block; (21 citations) Fc Block antibody (13 citations) CD16/32 antibody (9 citations) 2.4G2 antibody (2 citations)

2 protocols using cd16 32 antibody fc block 2.4g2

Multiparameter Flow Cytometry Analysis

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Cells from the lungs, tumors, and spleens were pre-incubated with CD16/32 antibody (Fc block; 2.4G2; BD Pharmingen) and stained with fluorochrome-conjugated antibodies from Biolegend: BV605-CD45 (30-F11), AF700-CD3 (17A2), PerCP/Cy5.5-CD4 (GK1.5), APC/Cy7-CD25 (PC61), PB-CD80 (16–10A1), APC-CD86 (GL-1), APC/Cy7-CD11c (N418), BV 650-IA/IE (M5/114.15.2), AF700-CD11b (M1/70), PerCP/Cy5.5-Gr-1 (RB6–8C5), PE-FOXP3 (150D), BV570-Ly6G (1A8), Pe/Cy7-Ly6C (HK1.4) as previously(15 (link)). Data were acquired on BD Fortessa and analyzed with FlowJo software (Tree Star).

Ghouse S.M., Vadrevu S.K., Manne S., Reese B., Patel J., Patel B., Silwal A., Lodhi N., Paterson Y., Srivastava S.K., Karbowniczek M, & Markiewski M.M. (2020). Therapeutic targeting of vasculature in the premetastatic and metastatic niches reduces lung metastasis. Journal of immunology (Baltimore, Md. : 1950), 204(4), 990-1000.

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Immune cell profiling of tumor samples

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Cells from tumors were pre-incubated with CD16/32 antibody (Fc block;
2.4G2; BD Pharmingen) and stained with fluorochrome-conjugated antibodies from
Biolegend: BV605-CD45 (30-F11), AF700-CD3 (17A2), PerCP/Cy5.5-CD4 (GK1.5), and
PE/Cy7-CD8a (53–6.7) as recommended by the manufacturer. To quantify
IFN-γ expression, cells were stained with surface markers and
permeabilized with Cytofix/Cytoperm (554714, BD Biosciences), and washed with 1x
Perm/Wash buffer (554714, BD Biosciences) followed by incubation with
PE-IFN-γ (XMG1.2, Biolegend). Prior to intracellular staining, cells were
incubated in the presence of brefeldin-A and monensin (BD Biosciences) in the
presence of CD3 and CD28 antibodies (17A2 and 37.51, eBioscience) adsorbed to
the 96-well plates for 6 to 8 h. Data were acquired on BD Fortessa and analyzed
with FlowJo software (Tree Star).

Reese B., Silwal A., Daugherity E., Daugherity M., Arabi M., Daly P., Paterson Y., Woolford L., Christie A., Elias R., Brugarolas J., Wang T., Karbowniczek M, & Markiewski M.M. (2020). Complement as Prognostic Biomarker and Potential Therapeutic Target in Renal Cell Carcinoma.. Journal of immunology (Baltimore, Md. : 1950), 205(11), 3218-3229.

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