Log In Sign up
The largest database of trusted experimental protocols

Matrigel coated transwell upper chambers

Manufactured by Corning
Visit Supplier
Sourced in United States

Matrigel-coated Transwell upper chambers are a laboratory product used for cell culture and migration studies. They feature a porous membrane coated with a basement membrane extract, Matrigel, to create a physiologically relevant microenvironment for studying cell behavior and migration.

Automatically generated - may contain errors

Lab products found in correlation

Image pro plus 6, by Media Cybernetics (1 mentions)

Close spelling variants of this product

Matrigel-coated upper chambers of Transwell inserts Matrigel-coated upper chamber Matrigel-coated Transwell chambers Upper transwell chamber Matrigel Transwell chambers Matrigel-coated chambers Coated transwell chambers Matrigel-coated Transwell Upper chamber Matrigel Chambers

2 protocols using matrigel coated transwell upper chambers

1

Transwell Invasion Assay for MKN-45 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
MKN‐45 cells were seeded into Matrigel‐coated Transwell upper chambers (#3491; Corning). After the cells were attached, the conditioned media were replaced with serum‐free media, and the chambers were placed into 24‐well plates with 600 μl media containing 20% FBS. Noninvaded cells were removed with a cotton swab, and the invaded cells were fixed with 4% paraformaldehyde and stained with crystal violet. Images were captured and analyzed by Image‐Pro plus 6.0 software (MediaCybernetics).
Chen J., Jin J., Gu W., Zhao Z., Yuan H., Zhou Y., Nagle D.G., Xi Q., Zhang X., Sun Q., Wu Y., Zhang W, & Luan X. (2023). Crizotinib‐based proteolysis targeting chimera suppresses gastric cancer by promoting MET degradation. Cancer Science, 114(5), 1958-1971.
+ Open protocol
+ Expand
2

CRC Cell Invasion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
CRC cells were added in Matrigel-coated transwell upper chambers (Corning, Cambridge, MA, USA) with serum-free medium, and lower chambers filled with DMEM plus 10% FBS. After 48 h, invaded cells were stained and then photographed under a microscope (Olympus) to obtain the numbers of invaded cells.
Bai L., Gao Z., Jiang A., Ren S, & Wang B. (2022). Circ_0001535 Facilitates Tumor Malignant Progression by miR-485-5p/LASP1 Axis in Colorectal Cancer. Balkan Medical Journal, 39(6), 411-421.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!