Anti vwf antibody

Manufactured by Merck Group

The Anti-vWF antibody is a laboratory reagent used for the detection and analysis of von Willebrand factor (vWF) in biological samples. Von Willebrand factor is a large glycoprotein involved in blood clotting and platelet adhesion. The Anti-vWF antibody can be utilized in various immunoassay techniques to quantify or characterize vWF levels and distribution in research and diagnostic applications.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Thrombin, by Merck Group (1 mentions) Alexa fluor 405, by Thermo Fisher Scientific (1 mentions) Methocult media, by STEMCELL (1 mentions) Fitc anti mouse cd41, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Adenosine diphosphate (adp), by Merck Group (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions) Sodium citrate buffer, by Abcam (1 mentions) Methyl green, by Vector Laboratories (1 mentions) Horse serum, by Vector Laboratories (1 mentions) Citrisolv, by Thermo Fisher Scientific (1 mentions) Immpact vip substrate, by Vector Laboratories (1 mentions) Fv1000, by Olympus (1 mentions) Hydroxyproline, by Merck Group (1 mentions) Formamide, by Merck Group (1 mentions) Ehrlich s reagent, by Merck Group (1 mentions)

5 protocols using anti vwf antibody

Immunophenotyping of Mouse Hematopoietic Stem Cells

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FITC-anti–mouse CD41, FITC-anti–human CD41, anti–mouse CD127, anti–mouse CD34, and anti–mouse CD41, FITC-anti–mouse c-Kit, APC-anti–mouse Sca-1, PE-CY7-anti–mouse CD16/32 antibodies, and eFluor450 lineage detection cocktail were purchased from eBioscience. PercP-CY5.5 goat anti–rat IgG and the antibodies against myc-tag, Mad2, CCP6, and GST-tag were purchased from Santa Cruz Biotechnology, Inc. The antibodies against Flag-tag, β-actin, and GFP-tag were from Sigma-Aldrich. Anti-VWF antibody was purchased from Millipore. GT335 (anti-glutamylation) antibody was from AdipoGen. Antibody against TTLL6 was from Novus Biologicals. The antibodies against Aurora B, Aurora A, pThr288 of Aurora A, tubulin, and CD42 were from Bioss. Anti-pThr232 antibody of Aurora B was from Antibodies Online. Alexa Fluor 405–, Alexa Fluor 488–, Alexa Fluor 594–, and Alexa Fluor 649–labeled secondary antibodies were from Molecular Probes. ADP, Thrombin, collagen, Hoechst 33342, and phorbol-12-myristate-13-acetate (PMA) were purchased from Sigma-Aldrich. TPO was from PeproTech. Aurora B kinase inhibitor AZD-1152 was from Selleck Chemicals. Methocult media were from STEMCELL Technologies.

Ye B., Li C., Yang Z., Wang Y., Hao J., Wang L., Li Y., Du Y., Hao L., Liu B., Wang S., Xia P., Huang G., Sun L., Tian Y, & Fan Z. (2014). Cytosolic carboxypeptidase CCP6 is required for megakaryopoiesis by modulating Mad2 polyglutamylation. The Journal of Experimental Medicine, 211(12), 2439-2454.

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Femoral Artery Histological Analysis

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Femoral arteries were perfusion fixed using paraformaldehyde, and then carefully excised along with a block of the surrounding muscle to save neighbor vasculature in the connective tissues. Excised tissues were then carefully fixed in 4% paraformaldehyde, embedded in the paraffin-block and serially sectioned. Cross-sections of the paraffin block were stained with hematoxylin and eosin (H&E) and Von Willebrand Factor (vWF) for endothelium staining. In briefly, for antigen retrieval, treatment dewaxed slides were heated in sodium citrate buffer (pH 6.0, abcam, Cambridge, MA) at 95 °C for 30 min in blocking solution of goat serum and incubated with the anti-vWF antibody (Millipore, Burlington, MA) at 4 °C overnight. Incubated slides were washed with PBS 3 times for 3 mins. The slides were incubated with Cy3-conjugated IgG (Jackson ImmunoResearch Laboratories, West Grove, PA) and counterstained with DAPI. The stained slides were observed by using a fluorescent microscope (IX-81, Olympus, Center Valley, PA). Brightness (+40%) and contrast (+40%) of acquired fluorescence images were adjusted to improve visibility by using ImageJ software^{43 (link)}.

Yu J., Lavery L, & Kim K. (2018). Super-resolution ultrasound imaging method for microvasculature in vivo with a high temporal accuracy. Scientific Reports, 8, 13918.

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Quantifying lung vasculature and muscularization

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Using a Leica Microtome RM2255 (Leica Biosystems, Buffalo Grove, IL), we made formalin-fixed and paraffin-embedded 5 μm slices of left lungs, deparaffinized with CitriSolv (Fisher Scientific, Pittsburgh, PA), and then rehydrated with a series of gradient ethanol. Sections were boiled in antigen unmasking solution; blocked using 10% horse serum (Vector Laboratories, Burlingame, CA); incubated overnight first with a mouse monoclonal α-smooth muscle actin (α-SMA) antibody, clone 1A4 (Sigma-Aldrich, St. Louis, MO), and then with peroxidase-conjugated anti-mouse/anti-rabbit secondary antibody (RTU Vectastatin Universal, Vector Laboratories, Burlingame, CA); and finally stained with peroxidase sensitive ImmPACT diaaminobenzidine substrate (Vector Laboratories, Burlingame, CA). Similarly, the von-Willebrand factor (vWF) was stained with rabbit polyclonal anti-vWF antibody (Sigma-Aldrich, St. Louis, MO) and peroxidase sensitive ImmPACT VIP substrate (Vector Laboratories, Burlingame, CA).^{32 (link)} The nuclei were counterstained with methyl green (Vector Laboratories, Burlingame, CA), and slides were viewed at 200× using the Olympus IX81 microscope (Olympus Scientific Solutions Americas Corp., Waltham, MA). ImageJ software analyzed the images, determined the degree of muscularization, and measured the medial wall thickness (MWT).

Rashid J., Nahar K., Raut S., Keshavarz A, & Ahsan F. (2018). Fasudil and DETA NONOate, Loaded in a Peptide-Modified Liposomal Carrier, Slow PAH Progression upon Pulmonary Delivery. Molecular pharmaceutics, 15(5), 1755-1765.

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Vascular Density Quantification by Anti-vWF Staining

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Capillary density was analyzed in the slice stained with anti-vWF antibody (1 : 200, Sigma). Cryosections were fixed with acetone for 30 minutes and endogenous peroxidase was quenched with 3% H₂O₂. After blocking with 2% normal goat serum, slices were incubated with the anti-vWF antibody at 4°C overnight. Then, FITC-conjugated IgG were incubated for 2 hours at room temperature before imaging under laser confocal microscope (FV1000, Olympus). The number of capillaries was calculated in five randomly chosen high magnification fields.

Liu J., Zhu P., Song P., Xiong W., Chen H., Peng W., Wang S., Li S., Fu Z., Wang Y, & Wang H. (2015). Pretreatment of Adipose Derived Stem Cells with Curcumin Facilitates Myocardial Recovery via Antiapoptosis and Angiogenesis. Stem Cells International, 2015, 638153.

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Comprehensive Analysis of Tissue Inflammation

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Albumin, anti-vWF antibody, 5-bromo-2-deoxyuridine (BrdU), chloramine Ttrihydrate, corn oil, Ehrlich’s reagent, Evans blue dye, formamide, hematoxylin and eosin (H&E), Hexadecyl trimethylammonium bromide, hydroxyproline, O-dianisidine dihydrochloride, trichrome kit, and trichloroacetic acid were purchase from Sigma-Aldrich (St Louis, MO). LPS from E. coli 055: B5 was obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX). RNeasy Mini kit, and DNase I were purchased from Qiagen USA (Germantown, MD). In Situ Cell Death Detection Kit, and SYBR Green master mix were obtained from Roche Diagnostics (Indianapolis, IN). Anti-BrdU antibody was purchased from BD Biosciences (San Jose, CA). DAPI, Alexa FITC-conjugated or Texas Red-conjugated secondary antibodies, and Trizol reagent were obtained from Thermo Fisher Scientific (Waltham, MA).

Wu C., Evans C.E., Dai Z., Huang X., Zhang X., Jin H., Hu G., Song Y, & Zhao Y.Y. (2017). Lipopolysaccharide-induced endotoxemia in corn oil-preloaded mice causes an extended course of lung injury and repair and pulmonary fibrosis: A translational mouse model of acute respiratory distress syndrome. PLoS ONE, 12(3), e0174327.

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