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Anti phospho iκb α monoclonal antibody

Manufactured by Cell Signaling Technology

The Anti-phospho-IκB-α monoclonal antibody is a laboratory reagent produced by Cell Signaling Technology. It is designed to specifically detect phosphorylated IκB-α protein, which plays a key role in the regulation of the NF-κB signaling pathway.

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2 protocols using anti phospho iκb α monoclonal antibody

1

Antibody Analysis of RIPK1 and RIPK3

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The following antibodies were used: anti-RIPK1 monoclonal antibody (BD Bioscience, 610459), anti-phospho-RIPK1 monoclonal antibody (Cell Signaling, #65746), anti-Flag monoclonal antibody (Sigma-Aldrich, F3165), anti-mouse-RIPK3 (Prosci, 2283), anti-phospho-IκB-α monoclonal antibody (Cell Signaling, 9246), and anti-β-actin monoclonal antibody (Sigma-Aldrich, A2066). The anti-human-RIPK3 polyclonal antibody was generated against full-length human RIPK3 recombination protein.
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2

Antibody Panel for Viral Protein Analysis

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The following antibodies were used: anti-VP16 monoclonal antibody (ab110226; Abcam), anti-gB monoclonal antibody (ab6505; Abcam), anti-β-actin monoclonal antibody (Sigma-Aldrich), anti-P65 polyclonal antibody (F0514; Santa Cruz), secondary antibody (Sigma-Aldrich), anti-phospho-IκB-α monoclonal antibody (9246; Cell Signaling), anti-Phospho-P65 monoclonal antibody (3033p; Cell Signaling), anti-phospho-JNK monoclonal antibody (9251; cell signaling), anti-ICP6 polyclonal antibody was generated in rabbit by immunization with recombinant ICP6 N-terminal polypeptide. Secondary antibody binding to Alexa Fluor 488 was purchased from Life Technologies.
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