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Hdl cholesterol

Manufactured by Roche
Sourced in Netherlands

HDL-Cholesterol is a laboratory test that measures the level of high-density lipoprotein (HDL) cholesterol in the blood. HDL is often referred to as 'good' cholesterol because it helps remove excess cholesterol from the body, reducing the risk of heart disease.

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2 protocols using hdl cholesterol

1

Lipid and Glucose Profiling Protocol

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Iodixanol was supplied as 60% w/v solution (OptiPrepTM) by Axis-Shield PLC (Dundee, UK). OptiSealTM tubes (3.2 ml) were supplied by Beckman Coulter (Krefeld, Germany). Triacylglycerol, Cholesterol, LDL-Cholesterol, HDL-Cholesterol, apoB, apoA1 and lipoprotein(a) assays, calibrators and serum lipid controls were purchased from Roche (Mannheim, Germany). For lipoprotein(a), the measuring range was 7–240 nmol/L (Tina-quant® Lipoprotein (a) Gen. 2, Roche). All lipid measurements were performed on the Cobas C501 from Roche. Inter- and intra-assay coefficients of variation for lipoprotein(a) are shown in Table S5. Non-HDL-C was calculated as the difference between TC and HDL-C. Glucose was measured on the Siemens ADVIA 2400. HbA1c was determined with the Tosoh G8 HPLC Analyzer. Hs-CRP was measured on the BN II Siemens nephelometric analyser. For fibrinogen, the ACL TOP (Instrumentation Laboratory) was used.
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2

Enzymatic Assays for Metabolic Markers

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Commercially available enzymatic kits were used to measure serum concentrations of triglycerides, total cholesterol and HDL-cholesterol (all Roche Diagnostics, Woerden, the Netherlands), free fatty acids (Wako chemicals, Nuess, Germany) and insulin (Meso Scale Diagnostics LLC, Rockville, MD, USA), and plasma glucose (Instruchemie, Delfzijl, the Netherlands). LDL-cholesterol was calculated by the Friedewald equation [16] .
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