Mmp 9 ab38898 antibody

The GAPDH (6C5) and β‐actin (C4) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The anti‐human ERα (D8H8), ERK1/2 (137F5), p‐ERK1/2 (197G2), AKT (11E7), p‐AKT (244F9), and p‐STAT3 (D3A7) antibodies for western blot were purchased from Cell Signaling Technology (Boston, MA, USA); MMP‐9 (ab38898) antibody was from Abcam (Cambridge, MA, USA). The anti‐mouse ERα (E115) for western blot was from Abcam. The CXCL12 (AF‐310‐NA) antibody for western blot was from R&D Systems (Minneapolis, MN, USA). Anti‐mouse/anti‐rabbit secondary antibody for western blot was from Invitrogen (Carlsbad, CA, USA). Normal rabbit IgG was also from Santa Cruz Biotechnology. MPP (CAS 911295‐24‐4) was from Bio‐Techne Corporation (Minneapolis, MN, USA). PMA (CAS 16561‐29‐8) and CCR2 antagonist (CAS 445479‐97‐0) were from MilliporeSigma (Burlington, MA, USA). AMD3100 (CAS 155148‐31‐5) was from Bio‐Techne Corporation. U0126 (CAS 109511‐58‐2) was from Cell Signaling Technology. MK‐2206 (CAS 1032350‐13‐2) and fulvestrant (CAS 129453‐61‐8) were from Selleckchem (Houston, TX, USA).

GAPDH (6c5), Pref-1, CD34, TGF-β1 and AR (N-20) antibodies were purchased from Santa Cruz Biotechnology (Paso Robles, CA). p-Smad3 and Smad3 antibodies were purchased from Cell Signaling Technology Company (Boston, MA), MMP-9 (ab38898) antibody was from Abcam Company (San Diego, CA). TGF-β type I receptor inhibitor (SB-431542) and MMP-9 specific inhibitor were from Cell Signaling Technology Company (Boston, MA). Crystal violet was from Fishers Scientific Company (Grand Island, NY). Anti mouse/rabbit second antibody for Western Blot and Lipofectamine 3000 transfection reagent were purchased from Life Technologies Company (Grand Island, NY). MiRNA-301a mimics and inhibitors were purchased from QIAGEN (Valencia, CA).