Itlc sg paper

Manufactured by Agilent Technologies

Sourced in United States

ITLC-SG paper is a type of thin-layer chromatography (TLC) material used for analytical and preparative separations. It is composed of silica gel coated on a flexible support, typically made of plastic or aluminum. The silica gel acts as the stationary phase, while the mobile phase, typically a solvent or solvent mixture, is used to separate the components of a mixture as they migrate through the ITLC-SG paper.

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Lab products found in correlation

Galliapharm, by Eckert & Ziegler (2 mentions) Dota noc, by Horiba (1 mentions) Hplc system, by Jasco (1 mentions) Alpha 1 2 ldplus freeze dryer, by Martin Christ (1 mentions) Minigita radio tlc scanner detector, by Elysia raytest (1 mentions) Radio tlc, by Bioscan (1 mentions)

Close spelling variants of this product

ITLC-SG (65 citations) ITLC-SG glass microfiber chromatography paper (8 citations) ITLC-SG chromatography paper (6 citations) ITLC-SG paper strips (2 citations)

6 protocols using itlc sg paper

Radiolabeling and Characterization of DOTA-NOC

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DOTA-NOC was procured from ABX, Germany. Sodium acetate and Suprapure HCl were purchased from Fluka, USA, and MERCK, Darmstadt, Germany, respectively. All reagents were prepared using sterile high-performance liquid chromatography (HPLC) grade water (Merck, India). Sterility test kits were obtained from Himedia Laboratories, India, and Endosafe PTS equipment and cartridges were from Charles River Laboratories Pvt. Ltd., India. ITLC SG paper was from Agilent Technologies, USA, whereas Whatman 3 mm chromatography strips were from Whatman, UK.
Radioactivity measurements were made using a NaI (Tl) counter (ECIL, India). An HPLC system (JASCO, Japan) equipped with a C-18 reversed phase column coupled to an ultraviolet/visible detector and a NaI (Tl) radioactivity detector (Raytest, Germany) was used for characterization of radiolabeled peptides. Alpha 1–2 LD plus freeze dryer was purchased from Martin Christ, GmBH while 0.22 μm membrane filters (33 mm) were from M/s. Millipore Corporation, Bedford, Massachusetts, USA. PET/CT studies were acquired on Philips Medical Systems, Ohio, USA GEMINI TOF 64.

Chandra P., Shetye B., Chakravarty R., Mukherjee A., Pandey U., Jha A.K., Purandare N., Shah S., Agrawal A., Ram R., Dash A, & Rangarajan V. (2017). Initial Clinical Experience with 68Ga-DOTA-NOC Prepared Using 68Ga from Nanoceria-polyacrylonitrile Composite Sorbent-based 68Ge/68Ga Generator and Freeze-dried DOTA-NOC Kits. World Journal of Nuclear Medicine, 16(2), 140-144.

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Radio-iTLC Analysis of [68Ga]Ga-NOTA-Insulin

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Radio-iTLC was performed using iTLC-SG paper
(Agilent Technologies, Santa Clara, CA). The paper was developed in
0.1 M sodium citrate solution (pH = 7) and analyzed on a radio-iTLC
scanner (Eckert & Ziegler, Valencia, CA). [⁶⁸Ga]Ga-NOTA-insulin R_f = 0.0–0.2, and free [⁶⁸Ga] R_f = 0.8–1.0.

Taubel J.C., Nelson N.R., Bansal A., Curran G.L., Wang L., Wang Z., Berg H.M., Vernon C.J., Min H.K., Larson N.B., DeGrado T.R., Kandimalla K.K., Lowe V.J, & Pandey M.K. (2022). Design, Synthesis, and Preliminary Evaluation of [68Ga]Ga-NOTA-Insulin as a PET Probe in an Alzheimer’s Disease Mouse Model. Bioconjugate Chemistry, 33(5), 892-906.

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Gallium-68 Labeling of RGD Dimer

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Gallium-68 chloride ([⁶⁸Ga]GaCl₃, 200 ± 41 MBq/500 µL) was obtained from the elution of a commercial TiO₂-based [⁶⁸Ge]Ge/[⁶⁸Ga]Ga generator (Galliapharm, Eckert & Ziegler, Berlin, Germany) using 0.1 N HCl, buffered with a fresh 4 mol L⁻¹ ammonium acetate solution (pH 7.4) and mixed with 10 μg of arginine-glycine-aspartate (RGD) dimer acetate precursor (RGD₂, ABX Chemicals, Radeberg, Germany), reaching a final pH of 6.0. The reaction mixture was stirred at room temperature for 10 min with gentle agitation. Radiochemical purity was determined by radio-thin layer chromatography (TLC) using a miniGITA radio-TLC scanner detector (Elysia-Raytest, Straubenhardt, Germany), iTLC-SG paper as solid phase (Agilent, Santa Clara, CA, USA), a 1:1 (v/v) mixture of 1 mol L⁻¹ aqueous ammonium acetate solution, and absolute methanol as mobile phase 1 (R_f [free ⁶⁸Ga]/[⁶⁸Ga]Ga-RGD₂: 0/1) and a 0.1 mol L⁻¹ sodium citrate pH 5 solution as mobile phase 2 (R_f [⁶⁸Ga]-RGD₂/[free ⁶⁸Ga]: 0/1). Radiosyntheses with radiochemical purities ≥95.0% were used.

Moyon A., Garrigue P., Balasse L., Fernandez S., Brige P., Bouhlel A., Hache G., Dignat-George F., Taïeb D, & Guillet B. (2021). Succinate Injection Rescues Vasculature and Improves Functional Recovery Following Acute Peripheral Ischemia in Rodents: A Multimodal Imaging Study. Cells, 10(4), 795.

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Radiolabeled Copper-Gold Nanoclusters for Targeted Cancer Imaging

Cited 1 time

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The ⁶⁴Cu (half-life = 12.7 h, β⁺ = 17%, β⁻ = 40%) was produced at the Washington University cyclotron facility with specific activity of 518 ± 281 GBq/μmol.^{48 (link), 72 (link) 64}CuAuNCs and ⁶⁴CuAuNCs-AMD3100 were prepared following the same procedure as for non-radioactive CuAuNCs. For the targeted nanocluster, a mixture of TA-PEG-OMe (2.5 mM, 400 μL) and TA-PEG-AMD3100 (2.5 mM, 200 μL) with molar ratio of 2:1 was used for surface pegylation. Instead of adding CuCl₂, radioactive ⁶⁴CuCl₂ (157 MBq) was added. The synthesized ⁶⁴CuAuNCs and ⁶⁴CuAuNCs-AMD3100 was then purified by centrifugation filtration (Amicon, 10K) and washed with phosphate buffer (pH = 7.4) three times. The radiochemical purity was determined by spotting 1 μL of solution on instant thin layer chromatography paper (iTLC SG paper, Agilent Technology) and developed in glass TLC chamber with 10% ammonium acetate + methanol (1:1 volume ratio) mixture as developing solution for radioactive thin layer chromatography analysis (Radio-TLC, BioScan). When the radiochemical purity was equal or greater than 95%, the ⁶⁴CuAuNCs and ⁶⁴CuAuNCs-AMD3100 would be diluted in saline (3.7 MBq/100 μL) for intravenous injection through tail vein.

Zhao Y., Detering L., Sultan D., Cooper M.L., You M., Cho S., Meier S.L., Luehmann H., Sun G., Rettig M., Dehdashti F., Wooley K.L., DiPersio J.F, & Liu Y. (2016). Gold Nanoclusters-Doped With 64Cu for CXCR4 Positron Emission Tomography Imaging of Breast Cancer and Metastasis. ACS nano, 10(6), 5959-5970.

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Radiolabeling of PSMA-11 with Gallium-68

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All chemicals and solvents were obtained from commercial suppliers and used without further purification. PSMA-11 precursor was purchased from ABX Advanced Compounds (Radeberg, Germany) and was diluted in a sodium acetate buffer solution (0.8 mol L⁻¹, pH = 4.5). PSMA-11 (2.5 µg, 250 µL) was added to Gallium-68 chloride ([⁶⁸Ga]GaCl₃, 100–200 MBq, 1 mL), which was previously eluted from a commercial [⁶⁸Ge]Ge/[⁶⁸Ga]Ga generator (Galliapharm, Eckert&Ziegler, Berlin, Germany). The radiolabeling was achieved after a 10-min incubation at 95 °C. Then, RCP was controlled by radio-thin layer chromatography (radio-TLC) using instant Thin Layer Chromatography–Silica Gel (iTLC-SG) paper (Agilent, Les Ulis, France) as the stationary phase, and a 1 mol L⁻¹ methanol/ammonium acetate solution (1:1; v/v) as the mobile phase.

Nail V., Louis B., Moyon A., Chabert A., Balasse L., Fernandez S., Hache G., Garrigue P., Taïeb D, & Guillet B. (2022). Sublingual Atropine Administration as a Tool to Decrease Salivary Glands’ PSMA-Ligand Uptake: A Preclinical Proof of Concept Study Using [68Ga]Ga-PSMA-11. Pharmaceutics, 14(6), 1276.

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Instant Thin Layer Chromatography for Radiochemical Analysis

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Instant Thin Layer Chromatography (ITLC) was conducted to determine the %LE and %RCY as described elsewhere [25, 34] . Briefly, ITLC-SG paper (Agilent Technologies, Forest Lakes, CA, USA) was used as stationary phase and spotted with the

Ebenhan T., Schoeman I., Rossouw D.D., Grobler A., Marjanovic-Painter B., Wagener J., Kruger H.G., Sathekge M.M, & Zeevaart J.R. (2017). Evaluation of a Flexible NOTA-RGD Kit Solution Using Gallium-68 from Different ⁶⁸Ge/⁶⁸Ga-Generators: Pharmacokinetics and Biodistribution in Nonhuman Primates and Demonstration of Solitary Pulmonary Nodule Imaging in Humans. Molecular imaging and biology, 19(3).

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