Clone rtk4530

Manufactured by BioLegend

The Clone RTK4530 is a laboratory equipment product offered by BioLegend. It serves as a core component in various research applications. The detailed specifications and intended use of this product are not available.

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Lab products found in correlation

Clone m1 70, by BioLegend (2 mentions) Sterile pbs, by Thermo Fisher Scientific (1 mentions) Clone sa214g2, by BioLegend (1 mentions) Hdm extract, by Greer Laboratories (1 mentions)

3 protocols using clone rtk4530

MSU Crystal-Induced Inflammation: Mechanisms and Resolution

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MSU crystals (3 mg) were injected intraperitoneally (i.p.) in mice and peritoneal exudates were collected at different time points (8, 24, 36 h) for the analysis of inflammatory cell composition. To examine the role of α_Mβ₂ integrin in MSU crystal-induced leukocyte recruitment, 50 μg of a blocking antibody against α_M integrin (clone M1/70; BioLegend) or an isotype control (clone RTK4530; BioLegend) were injected intravenously in mice 10 min prior to i.p. administration of the MSU-crystals. Mice were sacrificed and peritoneal exudates were analyzed 8 h after the MSU crystal injection.
The effect of DEL-1 on the regulation of resolution of MSU crystal-induced inflammation was investigated by injecting 5 μg of either DEL-1-Fc or Fc control i.p. 20 h after the treatment of mice with MSU crystals. 4 h after DEL-1-Fc injection, mice were sacrificed and clearance of apoptotic neutrophils was evaluated in peritoneal exudates.
In other experiments, 300 ng of RvD1 or vehicle control (PBS) were administered i.p. together with MSU crystals in wild-type and Del1^KO mice. 24 h after injection, the numbers of apoptotic neutrophils were measured in peritoneal exudates.

Kourtzelis I., Li X., Mitroulis I., Grosser D., Kajikawa T., Wang B., Grzybek M., von Renesse J., Czogalla A., Troullinaki M., Ferreira A., Doreth C., Ruppova K., Chen L.S., Hosur K., Lim J.H., Chung K.J., Grossklaus S., Tausche A.K., Joosten L.A., Moutsopoulos N.M., Wielockx B., Castrillo A., Korostoff J.M., Coskun Ü., Hajishengallis G, & Chavakis T. (2018). DEL-1 promotes macrophage efferocytosis and clearance of inflammation. Nature immunology, 20(1), 40-49.

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Intranasal House Dust Mite Challenge in Mice

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The mice received 3 intranasal installations of 20 μg (by total protein) of HDM extract (Greer Laboratories, Lenoir, NC) in 10 μL of sterile PBS (Gibco, Thermo Fisher, Waltham, Mass), without anaesthesia, from days 7 to 12 of life. A further 6 intranasal challenges of 25 μg of HDM extract in 15 μL of PBS were administered from days 14 to 27 of life under light isoflurane anaesthesia. HDM challenges were spaced 1 to 2 days apart 3 times per week, and equivalent volumes of PBS were used as controls where applicable. The mice were weaned at 20 to 23 days of age. In some experiments, rat anti-mouse blocking antibodies to CCR2 (clone MC-21,²⁷ (link) Matthias Mack Laboratory; 10 μg/dose), CCR8 (clone SA214G2, BioLegend, San Diego, Calif; 3 μg/dose), or equivalent doses of appropriate IgG2bκ isotype control antibodies (clone MC-67, Matthias Mack Laboratory and clone RTK4530, BioLegend for CCR2 and CCR8, respectively) were administered by intraperitoneal injection in a volume of 100 μL of PBS 24 hours before the seventh HDM challenge and then immediately before the seventh, eighth, and ninth HDM challenges. All analysis was performed 24 hours after the final HDM exposure.

Branchett W.J., Cook J., Oliver R.A., Bruno N., Walker S.A., Stölting H., Mack M., O’Garra A., Saglani S, & Lloyd C.M. (2021). Airway macrophage-intrinsic TGF-β1 regulates pulmonary immunity during early-life allergen exposure. The Journal of Allergy and Clinical Immunology, 147(5), 1892-1906.

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Investigating MSU Crystal-Induced Inflammation

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MSU crystals (3 mg) were injected intraperitoneally (i.p.) in mice and peritoneal exudates were collected at different time points (8, 24, 36 h) for the analysis of inflammatory cell composition. To examine the role of α_Μβ₂ integrin in MSU crystal-induced leukocyte recruitment, 50 μg of a blocking antibody against α_M integrin (clone M1/70; BioLegend) or an isotype control (clone RTK4530; BioLegend) were injected intravenously in mice 10 min prior to i.p. administration of the MSU-crystals. Mice were sacrificed and peritoneal exudates were analyzed 8 h after the MSU crystal injection.
The effect of DEL-1 on the regulation of resolution of MSU crystal-induced inflammation was investigated by injecting 5 μg of either DEL-1-Fc or Fc control i.p. 20 h after the treatment of mice with MSU crystals. 4 h after DEL-1-Fc injection, mice were sacrificed and clearance of apoptotic neutrophils was evaluated in peritoneal exudates.
In other experiments, 300 ng of RvD1 or vehicle control (PBS) were administered i.p. together with MSU crystals in wild-type and Del1^KO mice. 24 h after injection, the numbers of apoptotic neutrophils were measured in peritoneal exudates.

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