The hydroxyproline content in lung tissues was measured using Hydroxyproline (HYP) Assay Kit (Solarbio, BC0255). Briefly, tissues of equal weight were cut into as many pieces as possible. Then, tissues are baked overnight in 12 N hydrochloric acid at 110 °C and neutralized with sodium hydroxide. Next, the samples were added to 1.4% chloramine T in 0.5 M sodium acetate/10% isopropanol and incubated for 20 min at room temperature. Ehrlich's solution was added into samples for 30 min incubation at 65 °C. Finally, the absorbance values of all samples were measured at 560 nm, and the Hydroxyproline content was calculated based on the fresh weight of tissues and the standard curve.
Hydroxyproline hyp assay kit
Manufactured by Solarbio
Sourced in China
The Hydroxyproline (HYP) Assay Kit is a quantitative colorimetric assay used to determine the hydroxyproline content in various biological samples. Hydroxyproline is an amino acid found primarily in collagen, and its measurement can provide insights into collagen metabolism and turnover.
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Lab products found in correlation
2 protocols using hydroxyproline hyp assay kit
1
Hydroxyproline Quantification in Lung Tissue
2
Collagen Quantification in Lung Tissue
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The collagen concentration was measured by Hydroxyproline (Hyp) assay Kit (Solarbio life science, China) according to the manufacturer’s protocol. Some lung tissue samples were hydrolyzed in lysis buffer solution at 110 °C for 2h. Absorbance of colored products was measured at 560 nm. The Hyp content was calculated as micrograms of Hyp per milligram of wet weight (μg/mg).
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