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Sybr green premix pro taq hs qpcr tracking kit

Manufactured by Accurate Biology
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Sourced in China

The SYBR Green Premix Pro Taq HS qPCR Tracking Kit is a reagent designed for real-time quantitative PCR (qPCR) analysis. It contains a premixed solution of SYBR Green I dye, Taq DNA polymerase, and other necessary components for efficient and sensitive qPCR amplification and detection.

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Lab products found in correlation

Nanodrop 2000, by Thermo Fisher Scientific (2 mentions) Evo m mlv rt mix tracking kit, by Accurate Biology (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Primescript rt master mix, by Takara Bio (1 mentions) Lightcycler 96 real time pcr system, by Roche (1 mentions)

2 protocols using sybr green premix pro taq hs qpcr tracking kit

1

RNA Extraction and qPCR Analysis

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Total RNA from cells was extracted by Trizol (Invitrogen) following the manufacture's introduction. The concentration of RNA was measured with a Nanodrop‐2000 spectrophotometer (Thermo Scientific, USA). For analysis of mRNA expression, 1 µg total RNA was reverse transcribed using EvoM‐MLV RT Mix Tracking Kit (AG11734, Accurate Biotechnology, China), and qPCR was performed using SYBR Green Premix Pro Taq HS qPCR Tracking Kit (AG11733, Accurate Biotechnology, China). β‐actin was used as a housekeeping gene and relative expression of the target gene was determined using the 2 ‐ΔΔCt method.
Zou P., Lin R., Fang Z., Chen J., Guan H., Yin J., Chang Z., Xing L., Lang J., Xue X, & Chen M. (2023). Implanted, Wireless, Self‐Powered Photodynamic Therapeutic Tablet Synergizes with Ferroptosis Inducer for Effective Cancer Treatment. Advanced Science, 10(36), 2302731.
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2

Quantitative Gene Expression Analysis

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The cells were used to extract total RNA using a Total RNA Isolation Kit (BEI‐BEI Biotech). To analyse mRNA, cDNA was generated from total RNA by utilizing the reverse transcriptase PrimeScript® RT Master Mix (TaKaRa). The cDNA was employed for qPCR utilizing a Roche Light Cycler 96 Real‐time PCR System manufactured by Roche Diagnostics located in Switzerland. SYBR Green Premix Pro Taq HS qPCR tracking kit (AG11733) was purchased from Accurate Biotechnology Company (Changsha, China). The comparative ∆∆Ct method was utilized to calculate the relative expression with GAPDH as internal control. Table S3 contains the primer sequences.
Jing J., Ouyang L., Zhang H., Liang K., Ma R., Ge X., Tang T., Zhao S., Xue T., Shen J., Ma J., Li Z., Wu J., Yang Y., Zhao W., Zheng L., Qian Z., Sun S., Ge Y., Chen L., Li C, & Yao B. (2023). Omega‐3 polyunsaturated fatty acids and its metabolite 12‐HEPE rescue busulfan disrupted spermatogenesis via target to GPR120. Cell Proliferation, 57(2), e13551.
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