Excised tumors were embedded in optimal cutting temperature (OCT) compound, flash-frozen, sectioned using a Leica CM1900 Rapid Sectioning Cryostat, stained using anti-mouse F4/80 antigen eFluor 450 (clone BM8; eBioscience), and imaged using an upright Olympus BX63 microscope and a 100x oil-immersion objective. For immunohistochemistry, a biotinylated anti-rat IgG antibody was applied, and VECTASTAIN ABC kit (Vector Laboratories Inc.) along with a 3-amino-9-ethylcarbazole substrate (Dako) were used for color development. The sections were counterstained with Harris hematoxylin solution (Sigma-Aldrich) and scanned by using Nanozoomer 2.0RS (Hamamatsu, Japan). For immunofluorescence, anti-rat IgG-Alexa Fluor 405 (Abcam) as a secondary antibody and anti-mouse CD326 (EpCAM)-PE antibody (clone: G8.8, eBioscience) were used.
Anti mouse cd326 epcam pe antibody clone g8
Manufactured by Thermo Fisher Scientific
The Anti-mouse CD326 (EpCAM)-PE antibody (clone: G8.8) is a fluorescently labeled antibody that binds to the mouse CD326 (EpCAM) antigen. CD326 is a cell surface glycoprotein involved in cell-cell adhesion.
Automatically generated - may contain errors
Lab products found in correlation
Nanozoomer 2.0 rs, by Hamamatsu Photonics (2 mentions)
3 amino 9 ethylcarbazole substrate, by Agilent Technologies (2 mentions)
Anti rat igg alexa fluor 405, by Abcam (2 mentions)
Vectastain abc kit, by Vector Laboratories (2 mentions)
Bx63 microscope, by Olympus (2 mentions)
Harris hematoxylin solution, by Merck Group (1 mentions)
Harris haematoxylin solution, by Merck Group (1 mentions)
2 protocols using anti mouse cd326 epcam pe antibody clone g8
1
Cryosectioning and Immunostaining of Excised Tumors
2
Immunohistochemical Analysis of Tumor Tissue
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Excised tumours were embedded in optimal cutting temperature (OCT) compound, flash-frozen, sectioned using a Leica CM1900 Rapid Sectioning Cryostat (Leica), stained using anti-mouse F4/80 antigen eFluor 450 (clone BM8; eBioscience), and imaged using an upright Olympus BX63 microscope and a × 100 oil-immersion objective. For immunohistochemistry, a biotinylated anti-rat IgG antibody was applied, and VECTASTAIN ABC kit (Vector Laboratories Inc.) along with a 3-amino-9-ethylcarbazole substrate (Dako) were used for colour development. The sections were counterstained with Harris haematoxylin solution (Sigma-Aldrich) and scanned by using Nanozoomer 2.0RS (Hamamatsu). For immunofluorescence, anti-rat IgG-Alexa Fluor 405 (Abcam) as a secondary antibody and anti-mouse CD326 (EpCAM)-PE antibody (clone: G8.8, eBioscience) were used.
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