Bioluminescence imaging was carried out using the IVIS animal imaging system (Caliper Life Biosciences, Hopkinton, Massachusetts, USA) and analyzed using Living Image software. Ten minutes before imaging, mice were injected intraperitoneally with XenoLight d -Luciferin-K+ Salt Substrate (Perkin Elmer; Waltham, Massachusetts, USA) at 150 mg/kg. Mice were then anesthetized and imaged over a 1-min interval for 7–8 min. Using the Living Image software (Perkin Elmer), signal intensity was quantified within a defined region of the mouse and then the flux of photon counts quantified.
Xenolight d luciferin k salt substrate
Manufactured by PerkinElmer
Sourced in United States
XenoLight d-Luciferin-K+ Salt Substrate is a laboratory reagent used for in vitro bioluminescence assays. It serves as a substrate for luciferase enzymes, enabling the detection and quantification of luciferase-based reporter systems.
Automatically generated - may contain errors
2 protocols using xenolight d luciferin k salt substrate
1
Bioluminescence Imaging in Mice
2
Bioluminescent Imaging of Engineered MSCs
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Bioluminescent imaging was performed using an In vivo Imaging System (IVIS) (Perkin Elmer, Waltham, MA, USA). Briefly, animals were anaesthetised using a mixture of 2% isoflurane and 98% oxygen. To detect firefly luciferase expressed by huSkM MSCV-fluc-GFP, Xeno-light D-luciferin K+ salt substrate (Perkin Elmer, Waltham, MA, USA) was injected intraperitoneally (150 mg/kg body weight), and MSC EF1-mkate-nanoluc reporter was imaged using furimazine substrate (≈ 0.25 mg/kg, 40× dilution of Nano-Glo substrate, Promega, WI, USA) in 100 μL sterile PBS injected via the tail vein.
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