Anti pericentrin ab4448

The anti‐progerin (ab66587), anti‐lamin A (ab8980), anti‐aquaporin 1 (ab65837), anti‐Arl3b (ab136648), and anti‐pericentrin (ab4448) antibodies were purchased from Abcam. The anti‐lamin A/C antibody (for immunoblotting, customized) was generated by GeneTex (Taiwan). The anti‐emerin (sc‐15378), anti‐Arp2 (sc‐15389), anti‐WASP (sc‐8353), and anti‐CEP164 (used in Fig 7A, sc‐515403) antibodies were purchased from Santa Cruz Biotechnology. The anti‐acetylated tubulin (T6793), anti‐γ‐tubulin (T5326), anti‐TTBK2 (HPA018113), anti‐actin (A5441), and anti‐α–tubulin (T6199) antibodies were purchased from Sigma‐Aldrich. The anti‐nesprin 2 (PA5‐51933) and anti‐Arp3 (PA5‐80307) antibodies were purchased from Thermo Fisher Scientific. The anti‐CEP164 antibody (used in Fig 8A, 4533.00.02) was purchased from SDIX. The anti‐myosin Va antibody (NBP1‐92156) was purchased from Novus. The anti‐CP110 antibody (12780‐1‐AP) was purchased from ProteinTech. The anti‐keratin 8 antibody (AB 531826) was purchased from DSHB. The anti‐centrin antibody (04‐1624) was purchased from Merck Millipore. Phalloidin (conjugated with Alexa Fluor‐488 or ‐546), DNase I (conjugated with Alexa Fluor‐488), and fetal bovine serum (FBS) were purchased from Invitrogen. Cytochalasin D was purchased from Sigma‐Aldrich. SAG was purchased from Enzo.

Cell lysates were prepared in lysis buffer (150 mM NaCl, 1% NP40, 0.5% Na-deoxycholate, 1 mM EDTA, and 50 mM Tris-HCl; pH 7.4) with the addition of the 1X protease inhibitor cocktail (Roche Molecular Diagnostics) and 1X phosphatase inhibitor cocktail (P0044, Sigma). Anti-α-tubulin (DM1A), anti-γ-tubulin (GTU88), anti-actin, anti-GAPDH, anti-cyclin B, anti-phospho-Histone 3/serine 10, and anti-Aurora-B antibodies were purchased from Sigma. Anti-myc antibody was obtained from Invitrogen. Anti-pericentrin (Ab4448) was purchased from Abcam. Anti-GFP antibody (JL-8) was purchased from Clontech.