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Ampicillin amp

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Ampicillin (Amp) is a widely used antibiotic in laboratory settings. It is a beta-lactam antibiotic that inhibits the synthesis of the bacterial cell wall, effectively killing or stopping the growth of susceptible microorganisms.

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Lab products found in correlation

Kanamycin, by Carl Roth (1 mentions) Puregene yeast bacteria kit b, by Qiagen (1 mentions)

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Ampicillin (56 citations)

2 protocols using ampicillin amp

1

DC Depletion and Ampicillin Treatment

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For DC depletion, diphtheria toxin (DTx; Sigma-Aldrich) was injected 48 h after DCs+L.m. immunization, as described23 (link). Ampicillin (Amp; Carl-Roth GmbH) treatment was performed by injecting 1 mg i.p.
Kretschmer L., Flossdorf M., Mir J., Cho Y.L., Plambeck M., Treise I., Toska A., Heinzel S., Schiemann M., Busch D.H, & Buchholz V.R. (2020). Differential expansion of T central memory precursor and effector subsets is regulated by division speed. Nature Communications, 11, 113.
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2

Cultivation and DNA Extraction of Legionella

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The Legionella strains L. pneumophila JR32 (Sadosky, Wiater & Shuman, 1993 (link)) and ΔicmT (Segal & Shuman, 1998 (link)), L. longbeachae NSW150 and L. parisiensis DSM 19216 were grown in N-(2-acetamido)-2-aminoethanesulphonic acid (ACES) yeast extract (AYE) broth (Feeley et al., 1979 (link)) or on buffered charcoal yeast extract agar (Difco, Detroit, MI, USA) for three days at 37 °C. E. coli BL21 Star (DE3) (Novagen) was cultured in LB medium supplemented with 40 μg/mL kanamycin (Kan) and 100 μg/mL Ampicillin (Amp) (Carl Roth, Karlsruhe, Germany), if necessary. Cells were harvested and DNA was extracted using the Puregene Yeast/Bacteria Kit B (Qiagen) according to the manufacturer’s recommendations.
Tobias N.J., Ahrendt T., Schell U., Miltenberger M., Hilbi H, & Bode H.B. (2016). Legionella shows a diverse secondary metabolism dependent on a broad spectrum Sfp-type phosphopantetheinyl transferase. PeerJ, 4, e2720.
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