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Anti mouse cd38

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Anti-mouse CD38 is a laboratory reagent used for the detection and analysis of the CD38 protein expressed on the surface of mouse cells. It provides a tool for researchers to study the function and distribution of CD38 in various mouse biological systems.

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Lab products found in correlation

Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Gsk2578215a, by Bio-Techne (1 mentions) Egta am, by AnaSpec (1 mentions) Bapta am, by R&D Systems (1 mentions) Thapsigargin, by Bio-Techne (1 mentions) Chir99021, by Bio-Techne (1 mentions) Dorsomorphin, by Bio-Techne (1 mentions) Gsk2606414, by Bio-Techne (1 mentions) Naadp am, by AAT Bioquest (1 mentions) Rtk2758, by BioLegend (1 mentions) 8 br adpr, by Biolog (1 mentions) Lps e coli serotype r515, by Enzo Life Sciences (1 mentions) Zombie yellow viability stain, by BioLegend (1 mentions) Trustain fcx antibody, by BioLegend (1 mentions) Negative selection b cell isolation kit, by STEMCELL (1 mentions) Anti mouse alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Anti rabbit egfr, by Abcam (1 mentions) Mouse anti e cadherin, by Santa Cruz Biotechnology (1 mentions) Anti mouse cd206, by Abcam (1 mentions) Mouse anti vimentin, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Anti-CD38 (12 citations) FITC anti‐mouse CD38 (2 citations)

5 protocols using anti mouse cd38

1

Reagents for Immune Cell Stimulation

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The following antibodies were used for cell stimulation: purified anti-mouse CD38 (Clone 90; BioLegend, 102702), purified rat IgG2a, κ isotype control (Clone RTK2758; BioLegend, 400565). In certain experiments, the following reagents/inhibitors were used at the indicated concentration in cell culture: LPS (E. Coli Serotype R515; Enzo Life Sciences, ALX-581-007-L001), NAADP-AM (3 μM; AAT Bioquest, 21000), GSK2578215A (LRRK2 inhibitor, 1 µM; Tocris Bioscience, 4629), GPN (200 µM; Santa Cruz Biotechnology, sc-252858), EDTA (2 mM; Sigma-Aldrich, E6758), thapsigargin (500 nM; Tocris Bioscience, 1138), 8-Br-cADPR (100 µM; BIOLOG Life Science Institute, B065), 8-Br-ADPR (100 µM; BIOLOG Life Science Institute, B051), Ned-19 (100 µM; Cayman Chemical, 17527), cyclosporine (10 µM; R&D Systems, 1101/100), FK506 (5 nM; Eton Bioscience Inc, 1100060052), CHIR 99021 (GSK3B inhibitor, 5 µM; Tocris Bioscience, 4423), BAPTA-AM (10 µM; R&D Systems, 2787), EGTA-AM (10 µM; AnaSpec Inc, AS-84100), GSK2606414 (EIF2AK3/PERK inhibitor, 10 µM; Tocris Bioscience, 5107), and dorsomorphin (5 µM; Tocris Bioscience, 3093).
Nabar N.R., Heijjer C.N., Shi C.S., Hwang I.Y., Ganesan S., Karlsson M.C, & Kehrl J.H. (2021). LRRK2 is required for CD38-mediated NAADP-Ca2+ signaling and the downstream activation of TFEB (transcription factor EB) in immune cells. Autophagy, 18(1), 204-222.
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2

Isolation and Sorting of Germinal Center B Cells

Cited 1 time
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GC B cells were generated and sorted using a previously described protocol (Hwang et al., 2015 (link)). Briefly, 6–12-week-old M2A-GFP KI mice were immunized with sheep’s red blood cells. After 8–10 days, total B cells from the spleens and lymph nodes were isolated using the Negative Selection B cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions. Dead cells were stained using Zombie Yellow viability stain (BioLegend) and Fc receptors were blocked with the mouse TruStain FcX antibody (#156604). Cells were immunostained with anti-mouse CD38 (#102719), B220 (#103235), and GL-7 (#144617) purchased from BioLegend. GC B cells were sorted on a BD Aria III FACs sorter (Beckton Dickinson) for GFP+, Zombie Yellow-, B220+, CD38low and GL-7+ cells, and were used immediately.
Wang J.C., Yim Y.I., Wu X., Jaumouille V., Cameron A., Waterman C.M., Kehrl J.H, & Hammer J.A. (2022). A B-cell actomyosin arc network couples integrin co-stimulation to mechanical force-dependent immune synapse formation. eLife, 11, e72805.
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3

Immunofluorescence Microscopy of Cell Markers

Cited 1 time
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Immunofluorescence microscopy was performed to determine the TNFI, TCFI and PCC as previously described69 (link),70 (link). PCC values were determined by the strength of the relationship between two fluorochrome signals. Primary antibodies were: anti-rabbit-LSD1 (05–939; Merck Millipore), anti-goat-SNAI1 (sc-10433; Santa Cruz), anti-mouse-E-cadherin (sc-21791; Santa Cruz), anti-mouse-vimentin (sc-6260; Santa Cruz), anti-rabbit-PKC-θ-T538p (ab63365; Abcam), anti-rabbit-LSD1-s111p (ABE1462; Merck Millipore), anti-rabbit-ALDH1A1 (ab52492; Abcam), anti-rabbit-EGFR (ab2430; Abcam), anti-mouse-CSV (H00007431-M08; Abnova), anti-goat-ABCB5 (ab77549; Abcam), anti-rabbit-FAP-α (ab28244; Abcam), anti-goat CCL2 (sc-1304; Santa Cruz), anti-goat-F4/80 (sc-26642; Santa Cruz), anti-goat-CCR7 (NB100–712; Novus Biologicals), anti-mouse-CD38 (102761; Biolegend), anti-mouse-CD206 (ab8918; Abcam), and anti-goat-EGR2 (sc-204050; Santa Cruz). Secondary antibodies used: anti-rabbit-Alexa Fluor 488 (A21206; Life Technologies) or anti-rabbit-Alexa Fluor 568 (A10042; Life Technologies), anti-mouse-Alexa Fluor 568 (A10042; Life Technologies), or anti-goat-Alexa Fluor 633 (A21082; Life Technologies).
Boulding T., McCuaig R.D., Tan A., Hardy K., Wu F., Dunn J., Kalimutho M., Sutton C.R., Forwood J.K., Bert A.G., Goodall G.J., Malik L., Yip D., Dahlstrom J.E., Zafar A., Khanna K.K, & Rao S. (2018). LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer. Scientific Reports, 8, 73.
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4

Multiparametric Flow Cytometry Profiling

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Cell suspensions were incubated for 20 min at 4 °C in PBS containing 1% FCS and 10 mM EDTA with the following antibodies: anti-mouse CD45, anti-mouse CD31, anti-mouse PDPN, anti-mouse CD21/CD35, anti-mouse SCA1, anti-mouse B220, anti-mouse CD4, anti-mouse CD8, anti-mouse CD19, anti-mouse CD38, anti-mouse GL7 and anti-mouse CD11b (all from BioLegend); anti-mouse CD157 and anti-human CD45 (both from BD Biosciences); anti-human PDPN and anti-human CD31 (both from Thermo Fisher Scientific); and anti-human EPCAM, anti-human CD14, anti-human CD3 and anti-human CD19 (all from BioLegend). LIVE/DEAD cell discrimination was performed either by using a fixable BV510 Dead Cell Staining Kit (Molecular Probes) before antibody staining or by adding 7-aminoactinomycin D (Calbiochem) before acquisition. Cells were acquired with an LSR Fortessa (BD Biosciences) and analyzed with the FlowJo (v.10) software (FlowJo LLC) according to established guidelines. Cell sorting was performed using a BD FACSMelody Cell Sorter and the FACSChorus (v.1.3) software (BD Biosciences).
Lütge M., De Martin A., Gil-Cruz C., Perez-Shibayama C., Stanossek Y., Onder L., Cheng H.W., Kurz L., Cadosch N., Soneson C., Robinson M.D., Stoeckli S.J., Ludewig B, & Pikor N.B. (2023). Conserved stromal–immune cell circuits secure B cell homeostasis and function. Nature Immunology, 24(7), 1149-1160.
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5

Macrophage Phenotyping by Flow Cytometry

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106 cells in 100 μL FACS buffer were stained with fluorochrome-labelled antibodies for macrophage type-specific cell surface markers: anti-mouse CD23 (cat. # 101618, clone B3B4, Per.CP/Cy5.5, 7 μl, Biolegend, CA), anti-mouse CD38 (cat. # 102705, clone 90, FITC, 3 μl, Biolegend, San Diego, CA), anti-mouse CD80 (cat. # 104707, clone 16–10A1, PE, 3 μl, Biolegend, CA), anti-mouse CD206 (cat. # 141708, clone C068C2, APC, 3 μl, Biolegend, CA), anti-human CD80 (cat. # 305208, clone 2D10, PE, 10 μl, BD Pharmingen, CA), anti-human CD86 (cat. # 555660, clone 2331, APC, 10 μl, BD Pharmingen, CA), anti-human CD163 (cat. # 326512, clone RM3/1, PerCP-Cy5.5, 15 μl, BD Pharmingen, CA), anti-human CD206 (cat. # 551135, clone 19.2, FITC, 10 μl, BD Pharmingen, CA). Cell counts were obtained before flow staining by trypan blue exclusion and normalized to single stained and isotype controls on a FACSCanto II (BD Biosciences) or BD Accuri c6 (BD Biosciences) and FlowJo Ver. 10 software (Tree Star, Ashland, OR, USA) or BD FACS Xpress software were used for analyses.
Ganesan R., Henkels K.M., Shah K., de la Rosa X., Libreros S., Cheemarla N.R., Serhan C.N, & Gomez-Cambronero J. (2020). D-series Resolvins activate Phospholipase D in phagocytes during inflammation and resolution. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(12), 15888-15906.
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