The PARP inhibitors used in this study were purchased from Selleckchem (Houston, TX, USA) with the following names and catalog numbers: A-966492 (catalog number S2197), AG14361 (catalog number S2178), AZD2461 (catalog number S7029), E7449 (catalog number S8419), G007-LK (catalog number S7239), Niraparib (MK-4827; catalog number S2741), NMS-P118 (catalog number S8363), NU1025 (catalog number S7730), Olaparib (AZD2281, Ku-0059436; catalog number S1060), PJ34-HCl (catalog number S7300), Rucaparib (AG-014699, PF-01367338; catalog number S1098), Talazoparib (BMN673; catalog number S7048), and UPF1069 (catalog number S8038). Stock solutions were dissolved in dimethyl sulfoxide (DMSO) according to the manufacturer’s protocol to a concentration of 10 mM and stored at −20 °C. Table 1 lists the PARP inhibitors used in this study and their proposed targets.
Nu1025
Manufactured by Selleck Chemicals
Sourced in United States
NU1025 is a laboratory equipment product manufactured by Selleck Chemicals. It is a precision instrument designed for use in various scientific applications. The core function of NU1025 is to perform specific tasks required in a laboratory setting, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Niraparib mk 4827, by Selleck Chemicals (1 mentions)
Pj34 hcl, by Selleck Chemicals (1 mentions)
Azd2461, by Selleck Chemicals (1 mentions)
Ag14361, by Selleck Chemicals (1 mentions)
Upf1069, by Selleck Chemicals (1 mentions)
Rucaparib ag 014699 pf 01367338, by Selleck Chemicals (1 mentions)
Talazoparib bmn 673, by Selleck Chemicals (1 mentions)
G007 lk, by Selleck Chemicals (1 mentions)
A 966492, by Selleck Chemicals (1 mentions)
Nms p118, by Selleck Chemicals (1 mentions)
Sb 334867, by Selleck Chemicals (1 mentions)
Cck 8 kit, by Thermo Fisher Scientific (1 mentions)
Fbs dmem f 12 medium, by Thermo Fisher Scientific (1 mentions)
96 well plate, by Corning (1 mentions)
2 protocols using nu1025
1
PARP Inhibitor Compounds Evaluation
2
Orexin Receptor Antagonists and Neuronal Viability
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Hippocampal neurons were planted in 96-well plates (Corning, Corning, USA) for 24 h. Different reagents were added to cells for 24 h or 48 h. SB334867, an OX1 receptor (OX1R) antagonist [19 (link)], was obtained from Selleck Chemicals (Houston, TX, USA). TCSOX229, the OX2 receptor (OX2R) antagonist [20 (link),21 (link)], and orexin-A were purchased from MCE (NJ, USA). NU1025, an inhibitor of PARP [22 (link)], was obtained from Selleck Chemicals (Houston, TX, USA). The CCK-8 kit (Invitrogen, Carlsbad, CA, USA) mixed with free FBS DMEM/F-12 medium (Gibco, Carlsbad, CA, USA) was added to the wells after discarding medium. The plates were read using a microplate reader (Thermo, Waltham, MA, USA) at the wavelength of 490 nm.
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