IL-6 and TNF-α levels were measured by standard sandwich ELISA protocol from heart homogenates and serum. IL-6 was detected using a rat anti-mouse IL-6 monoclonal antibody (504502, BioLegend, San Diego, CA, USA) for capture and biotinylated rat anti-mouse IL-6 monoclonal antibody (504602, BioLegend, San Diego, CA, USA) for detection. TNF-α was measured using a rat anti-mouse TNF-α antibody (14-8321, eBioscience, San Diego, CA, USA) for capture and biotinylated rabbit anti-mouse TNF-α polyclonal antibody (14-7423, eBioscience, San Diego, CA, USA) for detection.
Bai Y.D., Yang Y.R., Mu X.P., Lin G., Wang Y.P., Jin S., Chen Y., Wang M.J, & Zhu Y.C. (2018). Hydrogen Sulfide Alleviates Acute Myocardial Ischemia Injury by Modulating Autophagy and Inflammation Response under Oxidative Stress. Oxidative Medicine and Cellular Longevity, 2018, 3402809.
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