Two independent anti-ASNS antibodies were used (ab40850, Abcam, San Francisco, CA; 50-560-171, Fisher Scientific, Pittsburgh, PA). Rabbit anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795-100UL), mouse anti–β-actin, and anti–α-amylase antibodies were purchased from Sigma-Aldrich (St. Louis, MO). ASNase (ab73439, Abcam; A3809, Sigma-Aldrich) was prepared as an aqueous stock at 1 U/μL concentration in water. Dolichos biflorus agglutinin-lectin (Vector Labs, Burlingame, CA), rabbit antibodies to phosphorylated PERK, PERK, phosphorylated p-eIF2α, and eIF2α were purchased from Cell Signaling Technology (Danvers, MA). Rabbit anti-ATF4 antibody and the reporter plasmid ASNS-173/+51/luciferase were kindly provided by Michael S. Kilberg. The latter was made by inserting an ASNS promoter fragment (nucleotides -173/+51) upstream of the Firefly luciferase reporter gene,26 (link) using the restriction site HindIII site of the luciferase reporter vector, pGL3 plasmid (Promega, Madison, WI).
Anti α amylase
Manufactured by Merck Group
Sourced in United States
Anti–α-amylase is a laboratory reagent used to inhibit the activity of the enzyme α-amylase. α-Amylase is an enzyme that breaks down starch into smaller carbohydrates. Anti–α-amylase can be used to study the role of α-amylase in various biological processes.
Automatically generated - may contain errors
2 protocols using anti α amylase
1
ASNS Protein Expression Regulation
2
Quantification of α-Amylase Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Saliva (15 μl) or tissue samples (200 μg wet weight) from wild-type (WT) and knockout (KO) mice were run on 4–12% SDS-PAGE gels, blotted into PVDF membranes, and probed with rabbit polyclonal anti-α-amylase (1:2,500 dilution; A8373, Sigma-Aldrich, St. Louis, MO, USA). Bound antibody was detected using WesternBreeze Chemiluminescent Western Blot Immunodetection Kit and BioMax Light film (Eastman Kodak Co., Rochester, NY, USA).
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