SOD activity and MDA concentration were measured by Total Superoxide Dismutase Assay Kit with WST-1 (Beyotime, China) and Lipid Peroxidation MDA Assay Kit (Beyotime, China), respectively. According to the manufacturer's protocols. Briefly, cells were washed twice with PBS then lysed by RIPA Lysis Buffer, cell debris were deleted through centrifuge, and the supernatant was used for continue assay. The absorbance of all samples was measured using a microplate reader (SOD, 450 nm; MDA, 530 nm) (6 (link)).
Total superoxide dismutase assay kit with wst 1
Manufactured by Beyotime
Sourced in China
The Total Superoxide Dismutase Assay Kit with WST-1 is a colorimetric assay designed to measure the total superoxide dismutase (SOD) activity in samples. The kit utilizes the water-soluble tetrazolium salt WST-1 as the indicator to detect the superoxide anion generated by the xanthine oxidase system.
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Lab products found in correlation
3 protocols using total superoxide dismutase assay kit with wst 1
1
Oxidative Stress Enzyme and Lipid Peroxidation Assay
2
Superoxide Dismutase Activity Assay
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The activity of antioxidant enzyme SOD in RLE-6TN cells or lung was determined by using a Total Superoxide Dismutase Assay Kit with WST-1 (Beyotime Biotechnology) according to the manufacturer’s protocol. Briefly, after cells or lung were washed, lysed and vortexed, the supernatant was extracted. Sample, WST and enzyme-working solutions were prepared and added into the 96-well plate. The mixtures were incubated at 37°C for 20 min., and the absorbance was finally determined at 450 nm using a microplate reader (SpectraMax M2) 21 (link).
3
Quantifying PAF-AH and SOD Levels
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The PAF-AH levels in the culture media were measured with a PAF Acetylhydrolase Kit (Cayman Inc., USA) following the manufacturer's instructions. The SOD levels in the cells were quantified with a Total Superoxide Dismutase Assay Kit with WST-1 (Beyotime Inc., China) according to the manufacturer's instructions.
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