Cholesterol ch

Cinnarizine (CIN) (CAS no. 298-57-7, Batch no. CIN/08161056) was kindly provided from Amoun pharmaceutical company, Egypt. Sorbitan monolaurate (span 20) was obtained from SDFCL, India. Cholesterol (CH) and sorbitan monostearate (span60) were procured from Loba Chemie, India. HPLC grades of chloroform, methanol, acetonitrile, ethanol and Millipore filter (0.45 µm) were obtained from Cornell lab, Cairo, Egypt. Chitosan (CS) (deacetylation degree ≥75% with low molecular weight) and mucin (from porcine stomach) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Analytical grades of glacial acetic acid (99%), potassium dihydrogen orthophosphate and disodium hydrogen phosphate were obtained from El-Nasr Pharmaceutical Chemical Co., Cairo, Egypt.

Phospholipid (phosphatidylcholine) was extracted from chicken egg yolk in the laboratory. Egg phosphatidylcholine (PC, purity > 96.5%) was obtained from Lipoid (Germany) to be used as a reference standard in the UHPLC analysis of extracted egg phospholipid (EP). Acetonitrile and HPLC grade Methanol were purchased from BDH Chemicals Ltd. (U.K.).
Black seed oil was bought from local market (Bangladesh). For quantification of amount of thymoquinone present in black seed oil, thymoquinone and Potassium dihydrogen phosphate were procured from Sigma-Aldrich (USA) and Winlab Ltd (UK) respectively. Cholesterol (Ch) was purchased from LobaChemie (India). Methanol, ethanol, dicholoromethane, acetone, BHT (Butylatedhydroxytoluene), sodium dihydrogen phosphate, and poly-ethylene glycol 6000 (PEG600) were obtained from Merck (Germany). Sodium chloride (NaCl) was obtained from Sigma-Aldrich (USA). All other reagents were of analytical grade and used without further purification.