The murine mAbs SARS2–02 and SARS2–38 studied were isolated from BALB/c mice immunized with SARS-CoV-2 spike and RBD proteins and have been described previously17 (link). Genes encoding CR3022, B38, and CV30 heavy and light chains were ordered from GenScript and cloned into pCMV/R. Antibodies were expressed by transient co-transfection of both heavy and light chain plasmids in Expi293F cells using PEI MAX (Polyscience) transfection reagent. Cell supernatants were harvested and clarified after 3 or 6 days and protein was purified using protein A chromatography (Cytiva). SPD-M162 was obtained from AcroBiosystems (AM122).
Protein a chromatography
Manufactured by Cytiva
Protein A chromatography is a technique used for the purification and isolation of antibodies and other proteins. It utilizes Protein A, a bacterial surface protein that has a high affinity for the Fc region of immunoglobulins. This method allows for the selective capture and separation of target proteins from complex mixtures.
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Lab products found in correlation
2 protocols using protein a chromatography
1
Isolation and Purification of SARS-CoV-2 Neutralizing Antibodies
2
Recombinant Antibody Expression Protocol
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The murine mAbs SARS2-02 and SARS2-38 studied were isolated from BALB/c mice immunized with SARS-CoV-2 spike and RBD proteins and have been described previously17 (link). Genes encoding CR3022, B38 and CV30 heavy and light chains were ordered from GenScript and cloned into pCMV/R. Antibodies were expressed by transient cotransfection of both heavy- and light-chain plasmids in Expi293F cells using PEI-MAX (Polyscience) transfection reagent. Cell supernatants were harvested and clarified after 3 or 6 days, and protein was purified using protein A chromatography (Cytiva). SPD-M162 was obtained from AcroBiosystems (AM122).
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