Primescript rt reagent kit with gdna eraser and tb green premix ex taq 2 tli rnaseh plus

LBPs (≥90%) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Potassium oxonate (97%) and allopurinol (≥99%) was purchased from Sigma-Aldrich Co. LLC (Shanghai, China). Uric acid (UA), serum creatinine (S_CR), blood urine nitrogen (BUN) and xanthine oxidase activity assay kit were purchased from Abcam Inc. (Cambridge, UK). The RNA isolation kit was obtained from Promega Biotechnology Co., Ltd. (Beijing, China). PrimeScript^TM RT reagent kit with gDNA Eraser and TB Green^® Premix Ex Taq™ II (Tli RNaseH Plus) was purchased from Takara Biomedical Technology Co., Ltd. (Dalian, China). Protease and phosphatase inhibitors were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Anti-OAT1, anti-OAT3 and β-actin antibody were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Anti-GLUT9 was purchased from Abcam Inc. (Cambridge, UK). The Amersham ECL System was purchased from GE Healthcare (Pittsburgh, PA, USA). Other biochemical reagents and chemicals were of analytical grade.

Dried flowers of Hemerocallis citrina Baroni were provided by Qidong Yunxing Lake Modern Agricultural Science and Technology Ecological Park Development Co., Ltd. (Qidong, Hunan, China). Anhydrous ethanol was purchased from Damao chemical reagent factory (Tianjing, China). Methanol and formic acid were purchased from Macklin (Shanghai, China). PRL and OT kit were purchased from Ruifan biotechnology company (Shanghai, China). Total RNA Kit was purchased from GBCBIO Technologies Inc. (Guangzhou, Guangdong, China). PrimeScriptTM RT reagent Kit with gDNA Eraser and TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) were purchased from Takara (Beijing, China).