Pkc γ

Manufactured by Addgene

PKC-γ is a protein kinase C isoform that is primarily expressed in the central nervous system. It plays a role in neuronal signal transduction and synaptic plasticity. The core function of PKC-γ is to phosphorylate target proteins, thereby modulating their activity and function.

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Lab products found in correlation

Pfuultra hf, by Agilent Technologies (2 mentions) Glun1a, by Addgene (2 mentions) Glun2a, by Addgene (2 mentions) Glun2b, by Addgene (2 mentions) Pkc γ dn, by Addgene (2 mentions) T4 dna ligase, by Thermo Fisher Scientific (2 mentions)

2 protocols using pkc γ

Constructing TRPM2 and NMDAR Plasmids

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GluN1a (Addgene, 17928), GluN2A (Addgene, 17924), GluN2B (Addgene, 17925), PKC-γ (Addgene, 112266), PKC-γ-DN (Addgene, 21239). The pcDNA4/TO-FLAG-hTRPM2 construct was a kind gift from Dr. Sharenberg AM (University of Washington, Seattle)(Perraud et al., 2003 (link)).
XbaI (BioLabs, R0145S), BamHI (BioLabs, R3136S), XhoI (BioLabs, R0146S), DpnI (BioLabs, R0176S), EcoRI (BioLabs, R3101S), KpnI (BioLabs, R3142S), NotI (BioLabs, R3189S) and T4 DNA ligase (Thermal Fisher Scientific, 2148085), PfuUltra HF (Agilent, 600380–51), and Q5® High-Fidelity DNA Polymerase (Biolabs, M0491S) were used to generating different deletion or mutation constructs.

Zong P., Feng J., Yue Z., Li Y., Wu G., Sun B., He Y., Miller B., Yu A.S., Su Z., Xie J., Mori Y., Hao B, & Yue L. (2022). Functional Coupling of TRPM2 and Extrasynaptic NMDARs Exacerbates Excitotoxicity in Ischemic Brain Injury. Neuron, 110(12), 1944-1958.e8.

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Molecular Manipulation of Ion Channels

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GluN1a (Addgene, 17928), GluN2A (Addgene, 17924), GluN2B (Addgene, 17925), PKC-γ (Addgene, 112266), PKC-γ-DN (Addgene, 21239), CKAR (Addgene, 14860). The pcDNA4/TO-FLAG-hTRPM2 construct was a kind gift from Dr. Sharenberg AM (University of Washington, Seattle).^{51 (link)} DpnI (BioLabs, R0176L) and PfuUltra HF (Agilent, 600380–51) were used to generating different deletion or mutation constructs.

Zong P., Feng J., Legere N., Li Y., Yue Z., Li C.X., Mori Y., Miller B., Hao B, & Yue L. (2024). TRPM2 enhances ischemic excitotoxicity by associating with PKCγ. Cell reports, 43(2), 113722.

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