Anti gr 1 clone rc57bl 6 8c5

Manufactured by Thermo Fisher Scientific

Anti-Gr-1 (clone RC57BL/6-8C5) is a monoclonal antibody that binds to the Gr-1 antigen, which is expressed on the surface of certain immune cells. It can be used for the identification and isolation of these cells in research applications.

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Lab products found in correlation

Lsrii flow cytometer, by BD (3 mentions) Hemavet 950, by Drew Scientific (2 mentions)

3 protocols using anti gr 1 clone rc57bl 6 8c5

Peripheral Blood Cell Immunophenotyping

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PB cell immunostaining was performed according to standard procedures, and samples were analyzed on a LSR II flow cytometer (BD Biosciences). For PB lineage analysis, the antibodies used were all from eBioscience: anti‐CD3ε (clone 145‐2C11), anti‐B220 (clone RA3‐6B2), anti‐Mac‐1 (clone M1/70), and anti‐Gr‐1 (clone RC57BL/6‐8C5). Lineage FACS analysis data are plotted as the percentage of B220⁺, CD3⁺, and Myeloid (Gr‐1⁺, Mac‐1⁺, and Gr‐1⁺ Mac‐1⁺) cells among total white blood cells. WBC, RBC, Ly, NE, Mo counts were generated by using the hemocytometer Hemavet 950, Drew Scientific Inc, (FL 33014), USA.

Florian M.C., Leins H., Gobs M., Han Y., Marka G., Soller K., Vollmer A., Sakk V., Nattamai K.J., Rayes A., Zhao X., Setchell K., Mulaw M., Wagner W., Zheng Y, & Geiger H. (2020). Inhibition of Cdc42 activity extends lifespan and decreases circulating inflammatory cytokines in aged female C57BL/6 mice. Aging Cell, 19(9), e13208.

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Analyzing Hematopoietic Cell Engraftment

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PB immunostaining was performed according to standard procedures, and samples were analyzed on a LSRII flow cytometer (BD Biosciences). Monoclonal antibodies to Ly5.2 (clone 104, eBioscience) and Ly5.1 (clone A20, eBioscience) were used to distinguish recipient from donor cells. For PB lineage analysis, the antibodies used were all from eBioscience: anti-CD3ε (clone 145-2C11), anti-B220 (clone RA3-6B2), anti-Mac-1 (clone M1/70), and anti-Gr-1 (clone RC57BL/6-8C5). The engraftment is plotted as percentage of donor-derived cells among total white blood cells. B cells, T cells, and myeloid cell lineage data are plotted as the percentage of B220⁺, CD3⁺, and Myeloid (Gr-1⁺, Mac-1⁺, and Gr-1⁺Mac-1⁺) cells among donor-derived cells. Please note that data are plotted in a logarithmic scale to accommodate sample heterogeneity.

Florian M.C., Klose M., Sacma M., Jablanovic J., Knudson L., Nattamai K.J., Marka G., Vollmer A., Soller K., Sakk V., Cabezas-Wallscheid N., Zheng Y., Mulaw M.A., Glauche I, & Geiger H. (2018). Aging alters the epigenetic asymmetry of HSC division. PLoS Biology, 16(9), e2003389.

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Murine Peripheral Blood Immune Profiling

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PB cell immunostaining was performed according to standard procedures and samples were analyzed on a LSRII flow cytometer (BD Biosciences). For PB lineage analysis the antibodies used were all from eBioscience: anti-CD3ε (clone 145-2C11), anti-B220 (clone RA3–6B2), anti-Mac-1 (clone M1/70), and anti-Gr-1 (clone RC57BL/6-8C5). Lineage FACS analysis data are plotted as the percentage of B220⁺, CD3⁺, and Myeloid (Gr-1⁺, Mac-1⁺, and Gr-1⁺ Mac-1⁺) cells among total white blood cells. WBC, RBC, Ly, NE, Mo counts were generated by using the hemocytometer Hemavet 950, DREW SCIENTIFIC Inc, (FL 33014), USA. More information on the antibodies used is provided in the Source Data file.

Montserrat-Vazquez S., Ali N.J., Matteini F., Lozano J., Zhaowei T., Mejia-Ramirez E., Marka G., Vollmer A., Soller K., Sacma M., Sakk V., Mularoni L., Mallm J.P., Plass M., Zheng Y., Geiger H, & Florian M.C. (2022). Transplanting rejuvenated blood stem cells extends lifespan of aged immunocompromised mice. NPJ Regenerative Medicine, 7, 78.

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