Anti mmp2 ab86607

Following heat treatment, cells were re-plated and cultured for an additional 24 h. Total cellular protein was extracted from the cell pellet and electrophoresed in 12% polyacrylamide gel and transferred to a nitrocellulose membrane [17 (link)]. The membrane was blocked in 5% skim milk and incubated with the following primary antibody at 4°C overnight: anti-ZEB1 (#70512; Cell Signaling Technology, Danvers, MA, USA), anti-Snail1 (PAB1924; Abnova, Taipei, Taiwan), anti-N-cadherin (#610921; BD Biosciences, Franklin Lakes, NJ, USA), anti-MMP2 (ab86607; Abcam, Cambridge, UK), and anti-GAPDH (MA5-15738; Thermo Fisher Scientific). Subsequently, the membrane was incubated with a secondary antibody conjugated with horseradish peroxidase at 37°C for 1 h. Chemiluminescent detection of proteins was captured with the Amersham ECL detection reagent (Cytiva, Marlborough, MA, USA).