The volatile oil of CX was obtained from Guangzhou Aroma Master Chenxiang Technology Co., Ltd. (Guangzhou, China) and the volatile oil of MX was obtained from Changshengyuan Medicinal Materials Development Co., Ltd. (Mianyang, China). Fluoxetine hydrochloride (FH) was purchased from Lilly Suzhou Pharmaceutical Co., Ltd. (Suzhou, China). The commercial enzyme-linked immunosorbent assay (ELISA) kits for adrenocorticotropic hormone (ACTH), corticosterone (CORT), 5-hydroxytryptamine (5-HT), dopamine (DA), norepinephrine (NE) and acetylcholine (Ach) were purchased from Jiangsu Enzymatic Immunity Industry Co., Ltd. (Yancheng, China). β-Actin and 5-HT1A antibodies were purchased from Abcam (Cambridge, UK). The reagents (i.e., ethanol, chloroform, isopropanol, H2O2 and xylene) were of analytical reagent grade and were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Horseradish peroxidase (HRP)-conjugated secondary antibodies were obtained from Biyuntian Biotechnology Co., Ltd. (Shanghai, China). The SYBR Green PCR kit was purchased from Thermo Fisher Scientific (China) Co., Ltd. Neutral gum and phosphate buffered saline (PBS) solution were obtained from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).
Phosphate buffered saline solution
Manufactured by Solarbio
Sourced in China
Phosphate-buffered saline (PBS) solution is a widely used buffer in biological and biochemical applications. It is a balanced salt solution composed of sodium phosphate, sodium chloride, and other components, providing a physiologically compatible pH and osmolarity. The primary function of PBS is to maintain the stability and integrity of biological samples, cells, or molecules during various experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
Neutral gum, by Solarbio (2 mentions)
Sybr green pcr kit, by Thermo Fisher Scientific (1 mentions)
Dialysis bag, by Solarbio (1 mentions)
Bca protein quantitation kit, by Thermo Fisher Scientific (1 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Isopropanol, by Thermo Fisher Scientific (1 mentions)
Ammonium hydroxide, by Thermo Fisher Scientific (1 mentions)
Neutral buffered formalin solution, by Solarbio (1 mentions)
5 protocols using phosphate buffered saline solution
1
Antidepressant Herb Oil Protocol
2
Synthesis and Characterization of Polymer-based Nanoparticles
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Titanium isopropoxide (TIP) (97%), pluronic block co-polymer F127, PEI (99%), BH, and dichloromethane were purchased from Shanghai Yien Chemical Technology Co., Ltd. (Shanghai, China). Anhydrous methanol and anhydrous ethanol were purchased from Tianjin Jiangtian Chemical Technology Co., Ltd. (Tianjin, China). The phosphate-buffered saline (PBS) solution and dialysis bag were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Sodium chloride was purchased from Tianjin Fengchuan Chemical Reagent Technology Co., Ltd. (Tianjin, China). Yeast extract power was purchased from Sinophar Chemical Reagent Co., Ltd. (Beijing, China). Ttyptone was purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). Ager was purchased from BioFroxx. Double distilled water (ddH2O) was purified from a Millipore purification system. E. coli was purchased from Tiangen Biotech (Beijing) Co., Ltd. (Beijing, China).
3
Preparation of H. pylori Lysate
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The H. pylori strain ATCC43504 (CagA+/VacA+), provided by the National Institutes for Food and Drug Control, was cultured on Columbia blood agar base medium (Oxoid, Hampshire, England)-coated plates supplemented with 5% defibrinated sheep blood (Fujian Sanqiang Biochemical Co., Ltd., Fujian, China) at 36 °C for 48 h under microaerophilic conditions (10% CO2). The H. pylori culture was harvested and then suspended in phosphate-buffered saline (PBS) solution (Solarbio, Beijing, China) and adjusted to a concentration of 6 × 106 CFU/mL.
The H. pylori suspension was placed on ice and sonicated for 30 s at 100 W power for a total of 10 times with intervals of 20 s each. Suspensions were centrifuged at 12,000×g for 10 min. The supernatant was removed, and the protein concentration was measured using a BCA protein quantitation kit (Thermo, MA, USA). The supernatant was diluted to 200 μg/mL with PBS and was considered H. pylori lysate.
The H. pylori suspension was placed on ice and sonicated for 30 s at 100 W power for a total of 10 times with intervals of 20 s each. Suspensions were centrifuged at 12,000×g for 10 min. The supernatant was removed, and the protein concentration was measured using a BCA protein quantitation kit (Thermo, MA, USA). The supernatant was diluted to 200 μg/mL with PBS and was considered H. pylori lysate.
4
Compound diphenoxylate and mosapride analysis
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Compound diphenoxylate tablets (batch number: 1712029) were purchased from Changzhou Kangpu Pharmaceutical Co., Ltd. (Jiangsu, China). Mosapride (batch number: 190201) was purchased from Jiangsu Haosen Pharmaceutical Co., Ltd. (Jiangsu, China). The commercial enzyme-linked immunosorbent assay (ELISA) kits for tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), substance P (SP), gastrin (GAS), 5-hydroxytryptamine (5-HT), and vasoactive intestinal peptide (VIP) were purchased from Jiangsu Enzymatic Immunity Industry Co., Ltd. (Yancheng, China). Neutral gum and phosphate-buffered saline (PBS) solution were obtained from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). HPLC-grade methanol, acetonitrile, isopropanol, ammonium hydroxide, and pure distilled water were obtained from Fisher Scientific (Fair Lawn, NJ, USA).
5
Chicken Tissue Sampling and Analysis
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At the end (day 42) of the experiment, chickens were deprived of feed for 12 h. Then 40 chickens (1 chicken per cage) were selected on treatment-wise average live weight to be representative, and slaughtered by cervical dislocation to collect a blood sample. The blood samples were separated for serum by centrifugation at 4°C, 3,000 rpm for 10 min. Serums were transferred into 1.5 mL microcentrifuge tubes, and nally stored at - 80°C for further examination. Immediately after slaughter, all segments of the small intestine, as well as cecum, were excised.
The gastrointestinal contents were collected from the cecum, hired in cryogenic vials, kept on dry ice and delivered to the laboratory and stored at - 80°C. Cecal contents were used for the detection of microbiota composition. For the sample of histological examination, 3 cm of small intestinal segment was taken from the middle of the duodenum, jejunum, ileum and cecum respectively, washed in phosphate buffered saline (PBS) solution to eliminate all the content and xed in 10% neutral buffered formalin solution (Solarbio, Beijing, P. R. China). For the assessment of the antioxidants in liver and breast muscles, tissues were collected into 1.5 mL Eppendorf tubes, then washed by PBS and transported to the laboratory on dry ice and frozen immediately in liquid nitrogen (- 80°C).
The gastrointestinal contents were collected from the cecum, hired in cryogenic vials, kept on dry ice and delivered to the laboratory and stored at - 80°C. Cecal contents were used for the detection of microbiota composition. For the sample of histological examination, 3 cm of small intestinal segment was taken from the middle of the duodenum, jejunum, ileum and cecum respectively, washed in phosphate buffered saline (PBS) solution to eliminate all the content and xed in 10% neutral buffered formalin solution (Solarbio, Beijing, P. R. China). For the assessment of the antioxidants in liver and breast muscles, tissues were collected into 1.5 mL Eppendorf tubes, then washed by PBS and transported to the laboratory on dry ice and frozen immediately in liquid nitrogen (- 80°C).
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