Viral nucleic acid extraction was performed using EZ1 Virus Mini Kit v2 (Qiagen, Hilden, Germany) on the EasyOne DNA isolation system (Qiagen). Bio-Speedy SARS-CoV-2 + Omicron RT-qPCR (Bioeksen R&D Technologies, Istanbul, Turkey) was employed for the detection of SARS-CoV-2 ORF1ab + N genes and NSP6 LSG105-107del mutation; PCR amplification and analysis were performed on a Bio-Rad CFX96 Real-time System (BioRad, Hercules, CA, USA). The samples with the cycle threshold (Ct) value < 33 for SARS-CoV-2 ORF1ab + N genes were considered positive for SARS-CoV-2 RNA. For the SARS-CoV RNA positive samples, if the difference between Ct values of NSP6 105-107/106-108del and Ct ofORF1ab + N genes is < 6, the sample is considered as an Omicron variant according to manufacturer instructions.
Easyone dna isolation system
Manufactured by Qiagen
Sourced in Germany
The EasyOne DNA isolation system is a lab equipment product designed for extracting and purifying DNA samples. It automates the DNA isolation process, allowing for efficient and consistent results. The core function of the EasyOne system is to isolate DNA from various sample types, preparing the samples for further analysis or downstream applications.
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Lab products found in correlation
3 protocols using easyone dna isolation system
1
SARS-CoV-2 and Omicron Detection
2
Genetic Profiling of Hereditary Disorders
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All individuals were subjected to genetic counseling and molecular testing. DNA was isolated from peripheral blood samples of patients by using the EasyOne DNA isolation system (Qiagen, Hilden, Germany). A custom-designed QIAseq Targeted DNA Panel (Qiagen, Hilden, Germany) was used for sequencing the entire coding region of ACVRL1, ADAM17, ENG, GDF2, PTPN14, RASA1, and SMAD4 genes. QCI analysis (Qiagen, Hilden, Germany) was used to control quality parameters, and Clinical Insight (Qiagen, Hilden, Germany) was used to determine variations. Variants were classified according to ACMG 2015 criteria (10 (link)).
3
Microbial Identification and SARS-CoV-2 Detection
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In our hospital, automated blood culture system (BacT/ALERT 3D system, bioMérieux, France) is used. For identification of the bacteria, conventional laboratory methods and Matrix-Assisted Laser Desorption Ionization Time-of-Flight (VITEK® MS system, bioMérieux, France) were used. Antimicrobial susceptibility test results were interpreted based on the European Committee on Antimicrobial Susceptibility Testing (EUCAST) recommendations. Besides, for defined cases that clinically requires an urgent result, lower respiratory tract samples (sputum, endotracheal aspirate specimens), were enrolled with BioFire® FilmArray® Pneumonia plus (bioMérieux, France).
As for SARS-CoV-2 RNA RT-PCR, nucleic acid extraction was performed using EZ1 Virus Mini Kit v2 (Qiagen, Hilden, Germany) on the EasyOne DNA isolation system (Qiagen, Hilden, Germany). Bio-Speedy SARS-CoV-2 þ Omicron RT-qPCR (Bioeksen R&D Technologies, Istanbul, Turkey) was used for the detection of SARS-CoV-2 ORF1ab&N genes; PCR amplification and analysis were performed on a Bio-Rad CFX96 Real-time System (BioRad, Hercules, CA, USA). The samples with the cycle threshold (Ct) value < 33 were considered positive for SARS-CoV-2 RNA.
As for SARS-CoV-2 RNA RT-PCR, nucleic acid extraction was performed using EZ1 Virus Mini Kit v2 (Qiagen, Hilden, Germany) on the EasyOne DNA isolation system (Qiagen, Hilden, Germany). Bio-Speedy SARS-CoV-2 þ Omicron RT-qPCR (Bioeksen R&D Technologies, Istanbul, Turkey) was used for the detection of SARS-CoV-2 ORF1ab&N genes; PCR amplification and analysis were performed on a Bio-Rad CFX96 Real-time System (BioRad, Hercules, CA, USA). The samples with the cycle threshold (Ct) value < 33 were considered positive for SARS-CoV-2 RNA.
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