Bone marrow mononuclear cells (MNCs) were first labelled with fluorescein isothiocyanate (FITC)-conjugated rat anti-mouse CD41 (BD). For ploidy analysis, murine Mks labelled with anti-CD41-FITC were fixed for 15 min at room temperature in 0.5% paraformaldehyde in PBS, permeabilized for 1 h at 4°C in 70% methanol, and stained with propidium iodide (PI). Polyploid murine Mks were those events with high forward scatter, positive CD41 expression and ≥16N DNA content.
Rat anti mouse cd41
Manufactured by BD
The Rat anti-mouse CD41 is a laboratory equipment used for the detection and analysis of the CD41 protein, which is a marker expressed on the surface of mouse platelets. This product can be utilized in various applications, such as flow cytometry and immunohistochemistry, to study the role of platelets in different physiological and pathological conditions.
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Lab products found in correlation
2 protocols using rat anti mouse cd41
1
Murine Megakaryocyte Ploidy Analysis
2
Comprehensive Immunophenotyping Using Diverse Antibodies
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The following primary antibodies were employed for immunohistochemistry, IF, and flow cytometry analysis: rat antimouse ICAM-1 (25ZC7) [31] (link), rat anti-mouse ICAM-2 (3C4) (BD Pharmingen), rat anti-mouse vascular cell adhesion molecule (VCAM-1) (2A11.12) [32] (link), rat anti-mouse PECAM-1 (Mec13.3) (BioLegend), rat anti-mouse PSGL-1 (4RA10) [33] (link), rat anti-mouse CD11b/Mac-1 (M1/70) (BD Pharmingen), rat anti-mouse CD4 (GK1.5) (BD Pharmingen), rat anti-mouse CD8 (Lyt2) (BD Pharmingen), rat anti-mouse CD41 (BD Pharmingen), rat anti-mouse CD45 (M1/9) (BD Pharmingen), rat CD45R/B220 (RA3-6B2) (BD Pharmingen), rat anti-mouse F4/80 (BD Pharmingen), rat antimouse 7/4 (AbD Serotec), rat anti-mouse Ly6G (1A8) [34] (link), rat anti-mouse Ter-119 (BD Pharmingen), rabbit anti-mouse Pselectin [35] (link), rabbit pan-laminin (DAKO Cytomation), rabbit Fig. 1 The flow chart summarizes the experimental setup and the number of mice analyzed at different stages of the study. The latter includes eight animals, which had to be excluded from further analysis. Additional 18 mice underwent sham surgery and are not listed here anti-fibrinogen (LSBio), goat anti-mouse CD3ε (Santa Cruz), and biotinylated murine IgG (Vector). Rat anti-human CD44 (huHERMES-1, 9B5) [36] (link), rat, goat, and rabbit IgG were applied as isotype controls.
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