Urea nitrogen

Manufactured by Nanjing Jiancheng

Sourced in China

Urea nitrogen is a type of laboratory equipment used to measure the level of urea, a waste product produced by the body, in a sample. It plays a crucial role in assessing kidney function and overall health. The core function of this equipment is to accurately determine the concentration of urea nitrogen in a given biological sample.

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Lab products found in correlation

Potassium oxonate, by Merck Group (1 mentions) Trizol, by Merck Group (1 mentions) Reagent kit, by Nanjing Jiancheng (1 mentions) Phosphate buffered saline (pbs), by Solarbio (1 mentions) Creatinine, by Nanjing Jiancheng (1 mentions) Benzbromarone, by Merck Group (1 mentions) Allopurinol, by Merck Group (1 mentions) Uric acid, by Merck Group (1 mentions) Anti glut9, by Bioss Antibodies (1 mentions) Anti il 1β antibody, by Proteintech (1 mentions) Anti tlr4, by Bioss Antibodies (1 mentions) Anti urat1, by Proteintech (1 mentions) Anti β actin, by Proteintech (1 mentions) Dihydroethidium, by Thermo Fisher Scientific (1 mentions) Calcein am propidium iodide, by Thermo Fisher Scientific (1 mentions) Hoechst 33258, by Thermo Fisher Scientific (1 mentions) Mmp 9, by Abcam (1 mentions) Aspartate aminotransferase, by Nanjing Jiancheng (1 mentions)

3 protocols using urea nitrogen

Uric Acid Regulation Pathway Assay

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TRIzol and potassium oxonate (PO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Allopurinol was purchased from Jiulian Co., Ltd (Hefei, China). Sodium carboxymethylcellulose (CMC) was purchased from Guichi Co., Ltd (Hangzhou, China). Phosphate-buffered saline was purchased from Solarbio Company (Beijing, China). Benzbromarone was purchased from Heumann Pharma GmbH (Nurnberg, Bavaria, Germany). Absolute ethyl alcohol was purchased from the fine chemical plant of the Laiyang Economic and Technological Development Zone (Yantai, Shandong Province, China). Trichloromethane was purchased from Shuangshuang Chemical Plant (Yantai, Shandong Province, China). Isopropanol was purchased from Fuyu Fine Chemical Industry (Tianjin, China). Diethyl pyrocarbonate (DEPC) was purchased from Sangon Biotech (Shanghai, China). A TRUEscript 1st strand cDNA synthesis kit and SYBR Green qPCR mix were purchased from Aidlab Biotechnologies Co., Ltd. (Beijing, China). Creatinine, urea nitrogen, and reagent kits were purchased from Nanjing Jiancheng Biological Company (Nanjing, China).

You W., Wang J., Zou Y., Che K., Hou X., Fei H, & Wang Y. (2019). Modified Chuanhu anti-gout mixture, a traditional Chinese medicine, protects against potassium oxonate-induced hyperuricemia and renal dysfunction in mice. The Journal of International Medical Research, 47(5), 1927-1935.

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Uric Acid Regulation via Inflammatory Pathways

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Uric acid, benzbromarone, allopurinol and PO were purchased from Sigma (St. Louis, USA). Elisa kits for IL-1β and TNF-α were purchased from Xinbosheng (Shenzhen, China). Anti-GLUT9, anti-TLR4 and anti-NLPR3 antibodies were purchased from Bioss (Beijing, China). Anti-OAT1, anti-β-actin, anti-URAT1, anti-Anti-MyD88 and anti-IL-1β antibodies were purchased from Proteintech (Chicago, USA). Xanthine oxidase, urea nitrogen and adenosine deaminase kits were purchased from Jiancheng (Nanjing, China).

Zhu J.X., Yang H.Y., Hu W.Q., Cheng J., Liu Y., Yi L.T, & Cheng H.Y. (2021). Active components from Lagotis brachystachya maintain uric acid homeostasis by inhibiting renal TLR4-NLRP3 signaling in hyperuricemic mice. Inflammopharmacology, 29(4).

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Photodynamic Therapy with DVDMS in Preclinical Studies

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DVDMS (molecular formula: C₆₈H₆₆N₈O₉Na₄, molecular weight: 1,230.265 Da, purity: >98%) was the property of Qinglong Hi-tech Co. Ltd. (Jiangxi, China). DVDMS was dissolved in phosphate-buffered saline (PBS) to a stock concentration of 2 mg/mL and stored in the dark at -20 °C.
3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltertrazolium bromide tetrazolium (MTT), singlet oxygen sensor green (SOSG), terephthalic acid (TA), fluorescein isothiocyanate-dextran (FD500), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), Dihydroethidium (DHE), Calcein-AM/propidium iodide (PI), Hoechst 33258, the fluorescent dyes 3,3-Dioctadecyloxacarbocyanine perchlorate (DiO), 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyaine iodide (DiR), mitochondrion tracker green (MTG), lysosome tracker green (LTG) and endoplasmic reticulum tracker green (ER) were purchased from Invitrogen (Thermo Scientific Inc., USA). Primary antibodies against proliferating cell nuclear antigen (PCNA) and matrix metalloproteinase-9 (MMP-9), were purchased from Abcam (Cambridge, UK). In situ cell death detection (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, TUNEL) kit was purchased from Roche. Test kits of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and urea nitrogen (BUN) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).

Liu Y., Bai L., Guo K., Jia Y., Zhang K., Liu Q., Wang P, & Wang X. (2019). Focused ultrasound-augmented targeting delivery of nanosonosensitizers from homogenous exosomes for enhanced sonodynamic cancer therapy. Theranostics, 9(18), 5261-5281.

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