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2k x 2k eagle ccd camera

Manufactured by Thermo Fisher Scientific
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The 2k x 2k Eagle CCD camera is a high-resolution digital imaging device designed for scientific and research applications. It features a 2048 x 2048 pixel sensor and is capable of capturing detailed images.

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Lab products found in correlation

Tecnai t20 microscope, by Thermo Fisher Scientific (3 mentions)

Close spelling variants of this product

Eagle 2k × 2k CCD camera Eagle 2k CCD camera Eagle 2K camera 2k × 2k CCD camera Eagle CCD camera CCD camera

3 protocols using 2k x 2k eagle ccd camera

1

Cryo-EM Structural Analysis of Proteins

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Proteins were diluted to approximately 0.02 mg/mL with buffer containing 10 mM HEPES, pH 7.0 and 150 mM NaCl, adsorbed to a freshly glow-discharged carbon-coated copper grid, washed with the same buffer, and stained with 0.7% uranyl formate. Datasets were collected using SerialEM (69 (link)) on an FEI Tecnai T20 microscope equipped with a 2k x 2k Eagle CCD camera and operated at 200 kV. The nominal magnification was 100,000, corresponding to a pixel size was 0.22 nm. Particles were selected from micrographs automatically using in-house written software (YT, unpublished), followed by manual correction using EMAN2 (70 (link)), when necessary. Reference-free 2D classifications were performed with Relion 1.4 (71 (link)).
Loomis R.J., DiPiazza A.T., Falcone S., Ruckwardt T.J., Morabito K.M., Abiona O.M., Chang L.A., Caringal R.T., Presnyak V., Narayanan E., Tsybovsky Y., Nair D., Hutchinson G.B., Stewart-Jones G.B., Kueltzo L.A., Himansu S., Mascola J.R., Carfi A, & Graham B.S. (2021). Chimeric Fusion (F) and Attachment (G) Glycoprotein Antigen Delivery by mRNA as a Candidate Nipah Vaccine. Frontiers in Immunology, 12, 772864.
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2

Cryo-EM Specimen Preparation and Imaging

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Samples were diluted with a buffer containing 20 mM HEPES, pH 7.0, 150 mM NaCl, adsorbed to a freshly glow-discharged carbon-film grid, washed with the above buffer, and stained with 0.7% uranyl formate. Images were collected semi-automatically at a magnification of 100,000 using SerialEM61 on a FEI Tecnai T20 microscope equipped with a 2k x 2k Eagle CCD camera and operated at 200 kV. The pixel size was 0.22 nm/px. Particles were picked manually using the swarm mode in e2boxer from the EMAN2 software package62 . Reference-free 2D classification was performed using EMAN2 and SPIDER63 .
Xu K., Acharya P., Kong R., Cheng C., Chuang G.Y., Liu K., Louder M.K., O’Dell S., Rawi R., Sastry M., Shen C.H., Zhang B., Zhou T., Asokan M., Bailer R.T., Chambers M., Chen X., Choi C.W., Dandey V.P., Doria-Rose N.A., Druz A., Eng E.T., Farney K., Foulds K.E., Geng H., Georgiev I.S., Gorman J., Hill K.R., Jafari A.J., Kwon Y.D., Lai Y.T., Lemmin T., McKee K., Ohr T.Y., Ou L., Peng D., Rowshan A.P., Sheng Z., Todd J.P., Tsybovsky Y., Viox E.G., Wang Y., Wei H., Yang Y., Zhou A.F., Chen R., Yang L., Scorpio D.G., McDermott A.B., Shapiro L., Carragher B., Potter C.S., Mascola J.R, & Kwong P.D. (2018). Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1. Nature medicine, 24(6), 857-867.
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3

Cryo-EM Specimen Preparation and Imaging

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Samples were diluted with a buffer containing 20 mM HEPES, pH 7.0, 150 mM NaCl, adsorbed to a freshly glow-discharged carbon-film grid, washed with the above buffer, and stained with 0.7% uranyl formate. Images were collected semi-automatically at a magnification of 100,000 using SerialEM61 on a FEI Tecnai T20 microscope equipped with a 2k x 2k Eagle CCD camera and operated at 200 kV. The pixel size was 0.22 nm/px. Particles were picked manually using the swarm mode in e2boxer from the EMAN2 software package62 . Reference-free 2D classification was performed using EMAN2 and SPIDER63 .
Xu K., Acharya P., Kong R., Cheng C., Chuang G.Y., Liu K., Louder M.K., O’Dell S., Rawi R., Sastry M., Shen C.H., Zhang B., Zhou T., Asokan M., Bailer R.T., Chambers M., Chen X., Choi C.W., Dandey V.P., Doria-Rose N.A., Druz A., Eng E.T., Farney K., Foulds K.E., Geng H., Georgiev I.S., Gorman J., Hill K.R., Jafari A.J., Kwon Y.D., Lai Y.T., Lemmin T., McKee K., Ohr T.Y., Ou L., Peng D., Rowshan A.P., Sheng Z., Todd J.P., Tsybovsky Y., Viox E.G., Wang Y., Wei H., Yang Y., Zhou A.F., Chen R., Yang L., Scorpio D.G., McDermott A.B., Shapiro L., Carragher B., Potter C.S., Mascola J.R, & Kwong P.D. (2018). Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1. Nature medicine, 24(6), 857-867.
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