We first fixed the cornea samples in a solution containing 2.5% glutaraldehyde and 4% PFA in a 0.1 M sodium cacodylate buffer pH 7.4. Then we fixed the corneal samples further with 1% osmium tetroxide and dehydrated them in a graded series of ethanol (30%, 50%, 70%, 85%, 95%) before critical point drying in a Tousimis Samdri-795 critical point drier. Before SEM imaging, we mounted the processed corneas to an aluminum SEM stub with carbon tape and silver paint and sputter-coated with 10 nm of Au/Pd in a Denton Desk IV sputter coater. Finally, we imaged all samples using a Hitachi SU8030 cFEG SEM at 10 kV.
Samdri 795 critical point drier
Manufactured by Tousimis
Sourced in United States
The Samdri-795 is a critical point drier manufactured by Tousimis. It is used to dry samples while preserving their original structure and morphology. The device utilizes the critical point drying process to remove liquid from the sample without causing damage or deformation.
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Lab products found in correlation
2 protocols using samdri 795 critical point drier
1
Corneal Ultrastructure Imaging by SEM
2
Ultrastructural Analysis of Decidual Tissue
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Decidual tissue with diameter of 1 mm from normal pregnant mice in the NP group and aborted mice of the A30E group were fixed in 2.5% glutaraldehyde for 2 h, then fixed in 1% osmic acid at 4 °C for 1 h. After washed with 0.1 M PBS, tissue samples were in turn dehydrated and with 50% ethanol, 70% ethanol, 90% ethanol, 100% ethanol (3 times), then ethanol:isoamyl acetate (1:1), and 100% isoamyl acetate. Samples were dried on a Samdri®-795 Critical point drier (Tousimis, U.S.A.) and sprayed with 10 nm metal conductive thin layer on an EMS 550 Ion beam sputtering coating instrument (EMS, U.S.A.). Samples were then observed and imaged under scanning electron microscope Teneo SEM (FEI, U.S.A).
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