All MICs were determined using serial two-fold microdilution method in 96-well microtiter plates as done previously68 (link). Antibiotics used in this study were: kanamycin (Kan; OmniPur), amikacin (Ami; ChemImpex), gentamicin (Gen; Sigma), tobramycin (Tob; Alfa Aesar), cefepime (Cef; ChemImpex), ciprofloxacin (Cip; Enzo), tigecycline (Tig; Astatech), imipenem (Imi; Combi-Blocks), and vancomycin (Van; Sigma). MIC values were determined at 18 hours post-inoculation by reading optical density at 600 nm (OD600) on a Synergy Neo2 Multi-Mode Plate Reader (Agilent BioTek), and inhibition of growth defined as OD600<0.5 above background. All MIC assays were performed at least twice starting from fresh streak plates on separate days and each assay was performed as two technical replicates. Assays were performed for all P. aeruginosa strains to determine starting MICs in the absence of berberine or berberine analogs. All MIC assays with berberine ligands (Ber, Ber-C3 [5 ], Ber-C12 [9 ], or Ber-pAr [10 ]) were adjusted for a final concentration of 1% DMSO in each well.
Imipenem imi
Manufactured by Combi-Blocks
Imipenem (Imi) is a broad-spectrum antibiotic used in research applications. It functions as a carbapenem, inhibiting bacterial cell wall synthesis. The product is supplied as a powder for reconstitution.
Automatically generated - may contain errors
Lab products found in correlation
Amikacin ami, by Chem-Impex (2 mentions)
Tobramycin tob, by Thermo Fisher Scientific (2 mentions)
Cefepime cef, by Chem-Impex (2 mentions)
Ciprofloxacin cip, by Enzo Life Sciences (2 mentions)
Synergy neo2 multi mode plate reader, by Agilent Technologies (2 mentions)
Gentamicin gen, by Merck Group (2 mentions)
Vancomycin van, by Merck Group (2 mentions)
2 protocols using imipenem imi
1
Antibiotic Susceptibility Profiling of P. aeruginosa
2
Antimicrobial Susceptibility Profiling of P. aeruginosa
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All MICs were determined using serial two-fold microdilution method in 96-well microtiter plates as done previously71 (link). Antibiotics used in this study were: kanamycin (Kan; OmniPur), amikacin (Ami; ChemImpex), gentamicin (Gen; Sigma), tobramycin (Tob; Alfa Aesar), cefepime (Cef; ChemImpex), ciprofloxacin (Cip; Enzo), tigecycline (Tig; Astatech), imipenem (Imi; Combi-Blocks), and vancomycin (Van; Sigma). MIC values were determined at 18 hours post-inoculation by reading optical density at 600 nm (OD600) on a Synergy Neo2 Multi-Mode Plate Reader (Agilent BioTek), and inhibition of growth defined as OD600<0.5 above background. All MIC assays were performed at least twice starting from fresh streak plates on separate days and each assay was performed as two technical replicates. Assays were performed for all P. aeruginosa strains to determine starting MICs in the absence of berberine or berberine analogs. All MIC assays with berberine ligands (Ber, Ber-C3 [5 ], Ber-C12 [9 ], or Ber-pAr [10 ]) were adjusted for a final concentration of 1% DMSO in each well.
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