CCK-8 assay (Abmole Bioscience, Shanghai, China) was used to measure GC viability in vitro. 3 × 103 cells were seeded in 96-well plates and grown in the incubator. At the indicated time, the complete medium used for cell cultivation was removed, and 10% CCK-8 solution diluted by the FBS-free medium was added into wells. The plates were incubated at 37 ℃ for 1 h protected from light. The absorbance of the CCK-8 solution at 450 nm was measured by a microplate reader (Biotek, VT, USA).
Cck 8 assay
Manufactured by AbMole
Sourced in China, United States
The CCK-8 assay is a colorimetric cell viability and cytotoxicity assay. It utilizes the tetrazolium salt WST-8 to measure the number of viable cells in a sample. The assay provides a simple, sensitive, and reliable method for determining cell proliferation, cell viability, and cytotoxicity.
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Lab products found in correlation
2 protocols using cck 8 assay
1
CCK-8 Assay for GC Cell Viability
2
Cell Viability Assessment by CCK-8 Assay
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HK-2 cells were plated in 96-well plates at 5×104 cells/well. After treatment, the viability of cells in each group was examined by CCK-8 assay (AbMole, USA). Briefly, each well was supplemented with 10 µL CCK-8 solution and incubated at 37 °C for 4 h, after which absorbance was measured at 450 nm using a microplate reader.
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