In vitro bacteriostatic and fungistatic activities of the macrocycles were evaluated against pathogenic representatives of Gram-positive bacteria (Staphylococcus aureus 209p, Bacillus cereus 8035), Gram-negative bacteria (Pseudomonas aeruginosa 9027, Escherichia coli F-50), and yeast (Trichophyton mentagrophytes var. gypseum 1773, Aspergillus niger BKMF-1119, Candida albicans 885–653). Minimal inhibitory concentrations (MICs) were estimated by conventional dilution methods for bacteria and fungi [27 (link)]. The antibacterial and antifungal assays were performed in Hottinger broth (HiMe-dia Laboratories Pvt. Ltd. Mumbai, India) and Sabouraud dextrose broth (HiMedia Laboratories Pvt. Ltd.) (bacteria 3 × 105 cfu/mL and yeast 2 × 104 cfu/mL). The bactericidal and fungicidal activities were determined as described earlier [28 (link)]. The tests were repeated three times.
Hottinger broth
Manufactured by HiMedia
Sourced in India
Hottinger broth is a culture medium used for the growth and isolation of microorganisms. It provides the necessary nutrients and growth factors required for the cultivation of various bacteria, fungi, and other microbes. The composition and formulation of Hottinger broth supports the optimal growth of a wide range of microbial species.
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Lab products found in correlation
2 protocols using hottinger broth
1
Antimicrobial Activities of Macrocycles
2
Antibacterial and Antifungal Evaluation
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In vitro antibacterial and antifungal activities of the cationic surfactant were evaluated against pathogenic representatives of gram-positive and gramnegative bacteria and fungi. Minimal inhibitory concentrations (MICs) were estimated by conventional dilution methods for bacteria and fungi [48] (link). The antibacterial and antifungal assays were performed in Hottinger broth (HiMe-dia Laboratories Pvt. Ltd Mumbai, India) and Sabouraud dextrose broth (HiMedia Laboratories Pvt. Ltd Mumbai, India) (bacteria 3 × 10 5 cfu/ml and yeast 2 × 10 4 cfu/ml). The components of Hottinger broth (Lactalbumin hydrolysate (10 g), Peptone (10 g), NaCl (5 g)) were dissolved in one liter of distilled water, autoclaved for 20 min at 121°C. The pH was adjusted to 7.2 prior to autoclaving. The components of Sabouraud dextrose (Peptone (10 g), glucose (40 g)) were dissolved in one liter of distilled water, autoclaved for15 min at 121°C. The pH was adjusted to 5.6 prior to autoclaving.
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