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Apramycin sulfate

Manufactured by Merck Group
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Apramycin sulfate is a laboratory reagent used in various research applications. It is a broad-spectrum aminoglycoside antibiotic that can be utilized for selective growth of bacteria in cell culture and other microbiology experiments.

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Lab products found in correlation

Kanamycin sulphate, by Merck Group (1 mentions) Agar, by Merck Group (1 mentions) Chloramphenicol, by Merck Group (1 mentions) Nalidixic acid, by Merck Group (1 mentions) Mgcl2, by Merck Group (1 mentions) Neomycin sulfate, by Thermo Fisher Scientific (1 mentions) N n dimethylformamide dmf dimethyl sulfoxide dmso, by Merck Group (1 mentions)

2 protocols using apramycin sulfate

1

Bacterial Conjugation Protocol with Selective Media

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Microorganisms used in the study were Escherichia coli ET12567/pUZ8002 58, (link)59 (link) , Escherichia coli ET12567/pUB307 58, (link)60 (link) For agar plates the media was prepared with 2% w/v of agar (Sigma-Aldrich; 05040). For conjugations, SFM media was supplemented to contain final concentration of 10 mM MgCl2 (Sigma-Aldrich; M1028). When selection was required, the medium was supplemented with following antibiotics and their final concentrations: 100 µg/mL apramycin sulfate (Sigma-Aldrich; A2024), 25 µg/mL chloramphenicol (Sigma-Aldrich; C0378), 50 µg/mL kanamycin sulphate (Sigma-Aldrich; K1377) and/or 25 µg/mL nalidixic acid (Sigma-Aldrich; N8878).
Maleckis M., Wibowo M., Gren T., Jarmusch S.A., Sterndorff E.B., Booth T., Henriksen N.N.S.E., Whitford C.M., Jiang X., Jørgensen T.S., Ding L, & Weber T. (2024). Biosynthesis of the Azoxy Compound Azodyrecin from Streptomyces mirabilis P8-A2. ACS chemical biology, 19(3).
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2

Synthesis of Acyl-modified Glycerol Ethers

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Paromomycin sulfate, neomycin sulfate, and 3.5kDa molecular weight cut-off (MWCO) dialysis membranes were purchased from Fisher Scientific. Apramycin sulfate, resorcinol diglycidyl ether (RDE), glycerol diglycidyl ether (GDE), butyryl (C4), hexanoyl (C6), octanoyl (C8), myristoyl (C14) or stearoyl chlorides (C18), triethylamine, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich. All other chemicals were purchased from Sigma-Aldrich and used without further purification.
Derakshani M., Lukow T, & Liesack W. (2001). Novel Bacterial Lineages at the (Sub)Division Level as Detected by Signature Nucleotide-Targeted Recovery of 16S rRNA Genes from Bulk Soil and Rice Roots of Flooded Rice Microcosms. Applied and Environmental Microbiology, 67(2), 623-631.
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