Spss 23.0 statistical software package

We present proportions and means for socio-demographic characteristics and technology use and perceptions. To assess differences between the adopter and non-adopter groups, we conducted chi-square tests of association for categorical variables and t-tests for continuous variables. For items capturing perceptions of technology adoption, we assessed Cronbach's alpha and created scales for each set of items. We used factor analysis to identify the factor structure of the items pertaining to perceived attributes of DOI theory. Given the exploratory nature of our study, our factor analysis was also exploratory and consisted of principal components analysis with varimax rotation and extraction based on eigenvalues greater than 1 and confirmed by examination of the scree plot. We reviewed the Kaiser-Meyer-Olkin measure of sampling adequacy and Bartlett's test of sphericity to ensure an appropriateness of factor analysis for the data. 20 Based on the results of the factor analysis, we created scales for the different factors using an average of the original data for the items comprising each scale. We employed the logistic regression analysis using a forced entry method to assess predictors of adoption of the patient portal and likelihood of using the portal. All analyses were conducted using the SPSS 23.0 statistical software package.

Descriptive statistics were calculated with Microsoft Office Excel 2007 (Microsoft, USA). Further statistical analysis was performed with spss 23.0 statistical software package (SPSS, USA). Student's t-test and Mann-Whitney U-test were used to compare continuous variables. A correlation between SOX11 promoter methylation and clinicopathological parameters was evaluated by Fisher's exact test. A P-value of <0.05 was considered statistically significant for all tests.

The apoptosis rate of 786-O and ACHN cells was determined via flow cytometry assays. Cells were added to a 6-well plate (3x10 5 cells/well) and subsequently transfected with-miR-181a-5p mimics or NC. Cells were collected 48 h post-transfection and washed with cold PBS (4˚C). Subsequently, the cells were resuspended in 100 µl 1X binding buffer, and 5 µl Annexin V-fluorescein isothiocyanate (Invitrogen; Thermo Fisher Scientific, Inc.) and 5 µl propidium iodide (Invitrogen; Thermo Fisher Scientific, Inc.) was added to each cell suspension. Cells were subsequently incubated at room temperature for 15 min in the dark, and 400 µl binding buffer was added to each tube. The apoptosis rate was determined using flow cytometry (EPICS, Xl-4; Beckman Coulter, Inc., Brea, CA, USA) and was analyzed with FlowJo software (version 10; Flow Jo LLC, Ashland, OR, USA).
Statistical analysis. Data are presented as mean ± standard error of the mean. All assays were repeated at least three times. Significance of differential expression was analyzed using one way analysis of variance followed by Tukey's post-hoc test. The SPSS 23.0 statistical software package (IBM Corp., Armonk, NY, USA) was used to perform statistical analysis. P<0.05 was considered to indicate a statistically significant difference.