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Nova pak hr c18 column

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The Nova-Pak HR C18 column is a high-resolution reversed-phase chromatography column designed for the separation and analysis of a wide range of compounds. It features a spherical silica-based stationary phase with a carbon-bonded C18 functionality, providing efficient and reproducible separation performance.

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Lab products found in correlation

343 spectropolarimeter, by PerkinElmer (1 mentions) Lc 6ad pump, by Shimadzu (1 mentions) Maxis q tof mass spectrometer, by Bruker (1 mentions) Avance 3 500 mhz spectrometer, by Bruker (1 mentions) Sephadex lh 20, by GE Healthcare (1 mentions) Rid 10a, by Shimadzu (1 mentions) Chirascan cd spectrometer, by Applied Photophysics (1 mentions) Silica gel 60, by Qingdao Marine Chemical (1 mentions) Bond elut c18 column, by Agilent Technologies (1 mentions) 996 photodiode array detector, by Waters Corporation (1 mentions) Q tof ms premier, by Waters Corporation (1 mentions) Acquity uplc system, by Waters Corporation (1 mentions)

Close spelling variants of this product

Nova-Pak C18 column (102 citations) Nova-Pak C18 (43 citations) Nova-Pak (14 citations) HR-C18 (2 citations) C18 column (384 citations)

3 protocols using nova pak hr c18 column

1

Synthesis and Characterization of EAK-YIGSR Peptide

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The synthesis, purification and characterization of EAK-YIGSR were performed as previously reported [29 (link)]. Briefly, the peptide EAK-YIGSR (sequence: H-Ala-Glu-Ala-Glu-Ala-Lys-Ala-Lys-Ala-Glu-Ala-Glu-Ala-Lys-Ala-Lys-Tyr-Ile-Gly-Ser-Arg-NH2) was synthesized on Rink Amide MBHA resin (0.52 mmol/g) using Fmoc chemistry by a Syro I synthesizer (Multisyntech, Witten, Germany). The side-chain protecting groups were: OtBu, Glu; Boc, Lys; Pbf, Arg; tBu, Ser and Tyr. All the couplings were double. After Fmoc-deprotection, the peptide was deblocked from the resin and deprotected from side-chain protecting groups using the mixture 1,9 mL TFA, 0.05 mL TES, 0.05 mL H2O, for 1.5 h. The resin was filtered off and the solution was concentrated. The product was precipitated with diethyl ether and filtered. The identity of the crude peptide was determined by MALDI mass spectrometry (exp. mass = 2192.06 Da; theor. mass = 2191.45 Da; AB-SCIEX TOF-TOF 4800 instrument). The peptide EAK-YIGSR was purified by RP-HPLC and characterized by analytical RP-HPLC (conditions: Nova-Pak HR C18 column (4 μm, 60 Å, 3.9 × 300 mm, Waters), eluent A: 0.05% TFA in H2O; eluent B: 0.05% TFA in CH3CN; gradient: from 18 to 26% di B in 24 min, flow rate: 1 mL/min; detector: 214 nm. tR = 10.68 min). The integration of the chromatogram gave a 99% purity grade.
Stocco E., Barbon S., Faccio D., Petrelli L., Incendi D., Zamuner A., De Rose E., Confalonieri M., Tolomei F., Todros S., Tiengo C., Macchi V., Dettin M., De Caro R, & Porzionato A. (2023). Development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration: A comparative study. Materials Today Bio, 22, 100761.
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2

Phytochemical Characterization of Natural Compounds

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Optical rotations were measured in MeOH on a Perkin-Elmer 343 spectropolarimeter. UV spectra and CD spectra were obtained simultaneously on a Chirascan CD spectrometer (Applied Photophysics Ltd., England) with MeOH as solvent. 1D NMR, and 2D NMR experiments were performed on a Bruker Avance III 500 MHz spectrometer. HR-ESIMS data were collected on a Bruker maXis Q-TOF mass spectrometer. Preparative and semipreparative HPLC were performed on a Shimadzu LC-6AD pump and a Shimadzu RID-10A refractive index detector with a Shimadzu Shim-packed Pro-ODS column (20 × 250 mm) and a Waters Nova-Pak® HR C-18 column (7.8 × 300 mm). Silica gel 60 (100–200 mesh, Qingdao Marine Chemical Ltd., Qingdao, China), YMC ODS (75 μm, YMC Co. Ltd., Kyoto, Japan) and Sephadex LH-20 (GE Healthcare, Uppsala, Sweden) were used in CC. Analytical TLC was performed on HSGF254 silica gel plates (0.2 mm, Yantai Jiangyou Silica Gel Development Co. Ltd., Yantai, China); spots were visualized by spraying with 10% H2SO4 solution followed by heating.
Yang L., Li X., Wu P., Xue J., Xu L., Li H, & Wei X. (2020). Streptovertimycins A–H, new fasamycin-type antibiotics produced by a soil-derived Streptomyces morookaense strain. The Journal of Antibiotics, 73(5), 283-289.
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3

Labeling Protocols for PET and SPECT Imaging

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Monosodium salt of 1-amino-8-naphthol-2, 4-disulfonic acid was purchased from TCI America, and all other chemicals were from Sigma-Aldrich. Mass spectra (MS) were obtained on a Waters Acquity UPLC system coupled with Waters Q-Tof Premier MS (liquid chromatography–mass spectrometry [LC-MS]). Semipreparative reversed-phase high-performance liquid chromatography (HPLC) was performed on a Waters 600 gradient system with a Waters 996 Photodiode Array detector using a Waters Nova-Pak HR C18 column (6 μm, 300 × 7.8 mm). Analytic reversed-phase HPLC was performed on a Perkin-Elmer Series 200 LC gradient system with a Waters 2784 Dual Absorbance ultraviolet detector plus a Bioscan radioisotope detector using a Waters Symmetry column (5 μm, 150 × 3.9 mm). The flow rate was 6 mL/min for the semipreparative column and 1 mL/min for the analytic column running the same linear gradient starting from 5% A (0.1% trifluoroacetic acid in acetonitrile) and 95% B (0.1% trifluoroacetic acid in water) for 5 min and increasing A to 65% at 35 min. A Varian Bond Elut C18 column (100 mg) was used for solid-phase extraction of the labeled product. 18F-fluoride and 64CuCl2 were obtained from the National Institutes of Health cyclotron facility.
Niu G., Lang L., Kiesewetter D.O., Ma Y., Sun Z., Guo N., Guo J., Wu C, & Chen X. (2014). In Vivo Labeling of Serum Albumin for PET. Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 55(7), 1150-1156.
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