HPSE-shRNA and control sh-NC were synthesized by Sigma-Aldrich; Merck KGaA. For the construction of the β-catenin vector, β-catenin cDNA was cloned into Flag-tagged-pcDNA3.1 (GenePharma). As regards the transfection of HPSE-shRNA, the transfection of control sh-NC and β-catenin-vector in PANC-1 and BxPC-3 cells was performed by Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) in accordance with the instructions of the manufacturer. The established stable cell lines were verified by RT-qPCR and western blot analyses and used for the subsequent experiments.
Control sh nc
Manufactured by Merck Group
Sourced in China
Control sh-NC is a laboratory equipment product. It serves as a control sample for evaluating the performance of short hairpin RNA (sh-RNA) experiments. The product provides a baseline reference to assess the effects of specific sh-RNA treatments.
Automatically generated - may contain errors
2 protocols using control sh nc
1
Construction and Validation of HPSE-shRNA and β-Catenin Vectors
2
Investigating DUSP14-Mediated β-Catenin Regulation
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DUSP14 shRNA (short-hairpin RNA) and control sh-NC synthesized by Sigma Company (Shanghai, China) were used in the experiments. For the construction of the β-catenin vector, we cloned the β-catenin cDNA into the Flag-tagged-pcDNA3.1 (GenePharma, China). For the transfection of the DUSP14-shRNA, guided by the manufacturer’s instructions, the controlled transfection of shNC and the β-catenin-vector in the PANC-1 and the BxPC-3 cell samples were carried out with Lipofectamine 2000 Transfection Reagent (Invitrogen, USA).
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