Gb11061 1

Manufactured by Wuhan Servicebio Technology

Sourced in China

The GB11061-1 is a laboratory equipment that serves as a thermal cycler. It is designed to perform polymerase chain reaction (PCR) amplification of DNA samples. The device precisely controls the temperature and duration of the thermal cycling process, which is a crucial step in the genetic analysis workflow.

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Lab products found in correlation

2 protocols using gb11061 1

Immune Cell Profiling of IL2RG and RAG KO Minipigs

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The spleens of IL2RG-KO minipigs, RAG-KO minipigs and age-matched wild-type minipigs were first fixed with 4% paraformaldehyde and then cut into paraffin sections. The sections were stained with H&E for histological analysis and analysed through immunohistochemistry (IHC). In IHC, rabbit anti-CD3 pAb (GB11014, Servicebio, China) was used to identify T lymphocytes, and rabbit anti-CD19 pAb (GB11061-1, Servicebio, China) was used to identify B lymphocytes. Rabbit anti-CD11b pAb (GB11058, Servicebio, China) was used to identify macrophages, neutrophil granulocytes and NK cells.

Chen B., Gu P., Jia J., Liu W., Liu Y., Liu W., Xu T., Lin X., Lin T., Liu Y., Chen H., Xu M., Yuan J., Zhang J., Zhang Y., Xiao D, & Gu W. (2019). Optimization Strategy for Generating Gene-edited Tibet Minipigs by Synchronized Oestrus and Cytoplasmic Microinjection. International Journal of Biological Sciences, 15(12), 2719-2732.

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Antibody Panel for Cellular Analysis

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The following antibodies were used in this research:
For flow cytometry analysis: anti-rat-CD3 (#201406, BioLegend), anti-rat-CD4 (#201509, BioLegend), anti-rat-CD8a (#201712, BioLegend), anti-rat-CD11b/c (#201817, BioLegend), anti-rat-granulocytes (#550002, BD Pharmingen), and anti-rat-CD68 (MCA341A700, Bio-Rad).
For immunohistochemistry: anti-myeloperoxidase (MPO) rabbit antibody (GB11224, Servicebio, 1:1,000), anti-CD68 rabbit antibody (GB11067, Servicebio, 1:500), anti-CD19 rabbit antibody (GB11061-1, Servicebio, 1:400), anti-CD3 rabbit antibody (GB111337, Servicebio, 1:1,000), anti-rat-endothelial-cell-antibody-1 (RECA-1, ab9774, Abcam, 1:200), and anti-CXCL1 (ab86436, Abcam, 1:200).
For immunocytochemistry: RECA-1 (ab9774, Abcam, 1:400), anti-CXCL1 (ab86436, Abcam, 1:100), and secondary antibodies (goat anti-mouse IgG Alexa Fluor^® 488, 1:200; goat anti-rabbit IgG Alexa Fluor 594^®, 1:200; ZSGB-BIO).
For Western blot: anti-p-p38 (#4511, Cell Signaling Technology, 1:1,000), anti-p38 (#8690, Cell Signaling Technology, 1:1,000), anti-p-p65 (#3033, Cell Signaling Technology, 1:1,000), anti-p65 (#8242, Cell Signaling Technology, 1:1,000), anti-GAPDH (#5174, Cell Signaling Technology, 1:2,000), anti-p47 (PA5-104250, Thermo Fisher Scientific, 1:2,000), and anti-p-p47 (PA5-99359, Thermo Fisher Scientific, 1:1,000).

Wang Y., Zhang Y., Liu Y., Xu J, & Liu Y. (2021). Gut–Liver Axis: Liver Sinusoidal Endothelial Cells Function as the Hepatic Barrier in Colitis-Induced Liver Injury. Frontiers in Cell and Developmental Biology, 9, 702890.

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