Uv 3600 shimadzuuv vis nir spectrometer

Lenvatinib
mesylate was purchased from Xi’an Qiyue Biotechnology Co. Ltd.
Pluronic F127, triethylamine, and THF were obtained from Shanghai
Aladdin Chemistry Co. Ltd. The singlet oxygen probe DPBF and ROS probe
DCFH-DA were purchased from Sigma-Aldrich Corporation. Nuclear stains
4,6-diamidino-2-phenylindole (DAPI) and MTT were supplied by Shanghai
Solarbio Co. Ltd.
Dulbecco’s modified Eagle’s
medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco.
Penicillin–streptomycin liquid (100×) was also obtained
from Gibco.
The UV–visible absorption was recorded by
a UV-3600 Shimadzu
UV–vis–NIR spectrometer (Shimadzu, Japan). Fluorescence
spectra were recorded by an F-4600 fluorophotometer (Hitachi, Japan).

Different
techniques were used to characterize the BDPBr₂ and BDPCl₂ and LBBr₂ NPs and LBCl₂ NPs. UV–vis–NIR
absorption spectra were recorded by a UV-3600 Shimadzu UV–vis–NIR
spectrometer (Shimadzu, Japan). Fluorescence spectra were recorded
by an F-4600 spectra fluorophotometer (Hitachi, Japan). Hydrodynamic
diameters and zeta potentials of LBPNPs were measured by Mastersizer
3000. (Zeta/DLS, Malvern, England). Characterizations of sample morphology
were performed on an S-4800 field-emission scanning electron microscope
(FESEM, Hitachi S4800, Japan) and a transmission electron microscope
(Jeol Jem-2010F, Japan). Taking the methoxyfluoroboron pyrrole BDPOMe
(BDPOMe, Φr = 0.30/Me CN) reported in the literature as the
reference standard,^{56 (link)} the reference method
was used to determine and calculate relative fluorescence quantum
yields of BDPBr₂, BDPCl₂, LBBr₂ NPs,
and LBCl₂ NPs. Time-correlated single photon counting was
used to determine the fluorescence lifetime of LBBr₂ NPs
and LBCl₂ NPs.