A commercially available multiplex suspension array system (Bio-Rad Laboratories) was used to quantify circulating concentrations of insulin, C-peptide, glucagon, leptin, resistin, visfatin, ghrelin, glucagon-like peptide 1, gastric inhibitory polypeptide, and plasminogen activator inhibitor-1. Plasma adiponectin concentrations were determined using a commercially available enzyme-linked immunosorbent assay (ELISA) kit (AdipoGen LifeSciences). Circulating concentrations of lipopolysaccharide binding protein (LBP; Biometec) and intestinal fatty acid-binding protein (iFABP; Ray- Biotech) were determined using commercially available ELISA kits. All assays were completed according to manufacturers’ instructions, and all samples were analysed in duplicate.
Multiplex suspension array system
Manufactured by Bio-Rad
The Multiplex suspension array system is a laboratory instrument designed for the simultaneous detection and quantification of multiple analytes in a single sample. It utilizes color-coded beads that are coated with specific capture reagents to enable the measurement of multiple targets in a small sample volume.
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Lab products found in correlation
2 protocols using multiplex suspension array system
1
Multiplex Biomarker Quantification
2
Cytokine Production in IgE-Stimulated BMMCs
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BMMCs were incubated overnight at 37°C with 1 µg/ml IgE anti-DNP in 1 ml culture medium and then stimulated for 24 h at 37°C with 10 ng/ml DNP-BSA. The amount of cytokine in the supernatant was determined using a Multiplex suspension array system (Bio-Rad Laboratories) or standard ELISA according to the manufacturer’s instructions.
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