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2 protocols using mab9521

1

Immunoblot and Immunofluorescence Antibody Protocol

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The following antibodies were used for immunoblot analysis and immunofluorescence labeling: anti-cathepsin C (dilution: 1:1000, sc-5647, Santa Cruz Biotechnology, RRID: AB_2086961), anti-trypsin (dilution: 1:1000, sc-137077, Santa Cruz Biotechnology, RRID: AB_2300318), anti-amylase (dilution: 1:1000, sc-46657, Santa Cruz Biotechnology, RRID: AB_626668), anti-cathepsin B (dilution: 1:1000, MAB965, R&D systems, RRID: AB_2086935), anti-cathepsin L (dilution: 1:1000, MAB9521, R&D systems, RRID: AB_2087829), anti-α/β-tubulin (dilution: 1:1000, #2148, Cell Signaling, RRID: AB_2288042), anti-Rab7 (dilution: 1:1000 (for Immunoblot) and 1:100 (for immunofluorescence), #9367, Cell Signaling, RRID: AB_1904103), anti-syncollin (dilution: 1:1000, ab178415, Abcam), anti-LAMPI (dilution: 1:1000, ab24170, Abcam, RRID: AB_775978), anti-GAPDH (dilution: 1:1000, H86504M, Meridian Life Science, RRID: AB_151542), anti-LIMPII (dilution: 1:1000, NB400-129, Novus Biologicals, RRID: AB_2301298), anti-LAMPII (dilution: 1:1000, L0668, Sigma Aldrich, RRID: AB_477154), anti-rat (dilution: 1:16,000, HAF005, R&D systems, RRID: AB_1512258), anti-goat (dilution: 1:16,000, sc-2020, Santa Cruz Biotechnology, RRID: AB_631728), anti-rabbit (dilution: 1:16,000 (for immunoblots) or 1:200 (for immunofluorescence), NA934V, Amersham) and anti-mouse (dilution: 1:16,000, NA931V, Amersham).
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2

Comprehensive Autophagy Protein Analysis

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The following antibodies were used: rabbit anti-C9ORF72 (sc-138763, Santa Cruz Biotechnology), rabbit anti-SMCR8 (ab121682, Abcam), rabbit anti-WDR41 (sc-137922, Santa Cruz Biotechnology), rabbit anti-ATG101 (SAB4200175, Sigma), rabbit anti-ATG13 (13468, Cell Signaling), rabbit anti-ULK1 (8054, Cell Signaling), rabbit anti–phospho-ULK1 (6888, Cell Signaling), rabbit anti-p62 (5114, Cell Signaling), rabbit anti-RFP (R10367, Invitrogen), mouse anti-LC3 (0231-100/LC3-5F10, Nanotools), rabbit anti-LC3 (PM036, MBL), mouse anti–cathepsin D (AF1029, R&D Systems), rat anti–cathepsin L (MAB9521, R&D Systems).
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