For immunoblotting, the following primary antibodies were used: anti‐ULK1 [clone D8H5] (Cell Signaling Technology, Rabbit MAb, Cat# 8054S, 1:1,000, RRID:AB_11178668), anti‐ATG14 [clone D1A1N] (Cell Signaling Technology, Rabbit MAb, Cat# 96752. 1:1,000, RRID:AB_2737056), pS29 ATG14 [clone D4B8M] (Cell Signaling Technology, Cat# 92340S, 1:1,000, RRID:AB_2800182); anti‐Vinculin [clone EPR 8185], (Abcam, Rabbit MAb, Cat# ab129002; 1:1,000, RRID:AB_2800182. Secondary antibodies for immunoblotting were purchased from Thermo Fisher Scientific. For immunocytochemistry, the following primary antibodies were used: anti‐ATPB [clone 3D5] (Abcam, Cat# ab14730; Mouse mAb, 1:500; RRID:AB_301438); anti‐BNIP3L/Nix [clone D4R4B] (Cell Signaling Technology, Cat# 12396, Rabbit mAb, 1:100; RRID:AB_2688036); anti‐ULK1 [clone D8H5] (Cell Signaling Technology, Rabbit mAb, Cat# 8054S, 1:500, RRID:AB_11178668); anti‐WIPI2 (Bio‐Rad, Cat# MCA5780GA, Mouse mAb, 1:500; RRID:AB_11178668). Secondary antibodies for immunocytochemistry were purchased from Invitrogen (Life Technologies) and used at 1:500. The DNA constructs used (Flag‐ULK1 [DU45617], mCherry‐GFP‐FIS 101‐152 [DU55502]) were generated at the MRC PPU Reagents and Services and are available to purchase online https://mrcppureagents.dundee.ac.uk.
Long M., Sanchez‐Martinez A., Longo M., Suomi F., Stenlund H., Johansson A.I., Ehsan H., Salo V.T., Montava‐Garriga L., Naddafi S., Ikonen E., Ganley I.G., Whitworth A.J, & McWilliams T.G. (2022). DGAT1 activity synchronises with mitophagy to protect cells from metabolic rewiring by iron  depletion. The EMBO Journal, 41(10), e109390.
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