Log In Sign up
The largest database of trusted experimental protocols

Ambion mmessage mmachine

Manufactured by Thermo Fisher Scientific
Visit Supplier

The Ambion mMessage mMachine is a high-performance in vitro transcription system for the synthesis of capped and polyadenylated mRNA from a DNA template. It provides a streamlined workflow for generating mRNA for a variety of applications, including gene expression, protein production, and RNA-based therapeutics.

Automatically generated - may contain errors

Lab products found in correlation

Mirna mimic, by Thermo Fisher Scientific (1 mentions) Dmem medium, by Takara Bio (1 mentions)

Close spelling variants of this product

Ambion mMESSAGE mMACHINE T7 Transcription Kit Ambion mMESSAGE mMACHINE SP6 Transcription Kit Ambion mMESSAGE mMACHINE SP6 kit Ambion T7 mMESSAGE mMACHINE kit Ambion mMessage mMachine kit MMessage mMachine

2 protocols using ambion mmessage mmachine

1

3T3 Fibroblast Transfection with Bcan 3'UTR and miR-9

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
3T3 fibroblast were purchased from ATCC and grown in DMEM medium with tetracycline-free 10% FBS (Clontech). For transfection we used the Bcan 3′UTR plasmid or miR-9 GFP sensors (1 mg ml−1) along with miRNA mimics (Thermo Scientific; 500 nM each, Supplementary Table 4) and in combination with mCherry RNA. To generate mRNA for transfection, we used the pSLU plasmid64 (link) and the Ambion mMessage mMachine (AM1345; Ambion) according to manufacturer’s instructions.
Wohl S.G., Jorstad N.L., Levine E.M, & Reh T.A. (2017). Müller glial microRNAs are required for the maintenance of glial homeostasis and retinal architecture. Nature Communications, 8, 1603.
+ Open protocol
+ Expand
2

Gata2a and Gata2b mRNA synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Full length pCS2+:Gata2a was a gift from Barry Paw. The coding sequence of Gata2b was PCR amplified from embryo RNA using Superscript III Platinum one-step PCT kit (Invitrogen, Thermo Fisher Scientific), and TOPO cloned into pcDNA3.1V5-His. Forward orientation was confirmed by sequencing. mRNA was synthesized using Ambion mMessage mMachine (Ambion, Thermo Fisher Scientific) Sp6 or T7 ultra kit, respectively.
Peña O.A., Lubin A., Rowell J., Hoade Y., Khokhar N., Lemmik H., Mahony C., Dace P., Umamahesan C, & Payne E.M. (2021). Differential Requirement of Gata2a and Gata2b for Primitive and Definitive Myeloid Development in Zebrafish. Frontiers in Cell and Developmental Biology, 9, 708113.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!