Yeast extract, potato dextrose agar, agar, and sucrose were purchased from Biolife (Milan, Italy). Ethylenediaminetetraacetic acid disodium salt (EDTA-2Na) was from Pharmacia Biotech (Uppsala, Sweden), stabilized 3% solution of hydrogen peroxide (H2O2) was obtained from Fluka (Buchs, Switzerland), and potassium cyanide was purchased from Sigma Aldrich (Taufkirchen, Germany). Hydrochloric acid as purchased from Merck (Darmstadt, Germany). Trichloroacetic acid (TCA) and ascorbic acid were from Kemika (Zagreb, Croatia), absolute ethanol was from Panreac (Barcelona, Spain), while butylated hydroxytoluene (BHT) and 2-thiobarbituric acid (TBA) was obtained from Acros Organics (Morris Plains, New Jersey, USA). Superoxide dismutase from bovine erythrocytes (3000 U mg −1 protein) (SOD) and xanthine oxidase from bovine milk (0.4-1.0 U mg −1 protein) (XOD) were purchased from Sigma-Aldrich (Taufkirchen, Germany). Formic acid was purchased from Fluka (Buchs, Switzerland). Aflatoxin standard mix (B1, B2, G1, G2) was purchased from Biopure (Tulln, Austria). Acetonitrile and methanol (both HPLC grade) were purchased from J. T. Baker (Radnor, PA, USA).
2 thiobarbituric acid
Manufactured by Thermo Fisher Scientific
Sourced in Spain
2-thiobarbituric acid is a chemical compound that is commonly used in analytical chemistry and biochemistry. It is a white crystalline solid that is soluble in water and organic solvents. The primary function of 2-thiobarbituric acid is to serve as a reagent in the detection and quantification of lipid peroxidation, a process that occurs in biological systems and is often associated with oxidative stress.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Merck Group (3 mentions)
Potassium cyanide, by Merck Group (2 mentions)
Xanthine oxidase from bovine milk, by Merck Group (2 mentions)
Butylated hydroxy toluene, by Thermo Fisher Scientific (2 mentions)
Ethylenediaminetetraacetic acid disodium salt edta 2na, by GE Healthcare (2 mentions)
Absolute ethanol, by ITW Reagents (2 mentions)
Sucrose, by Biolife (1 mentions)
Agar, by Biolife (1 mentions)
Superoxide dismutase from bovine erythrocytes, by Merck Group (1 mentions)
Ascorbic acid, by Kemika (1 mentions)
Yeast extract, by Biolife (1 mentions)
Potato dextrose agar (pda), by Biolife (1 mentions)
Trichloroacetic acid, by Kemika (1 mentions)
Ethylenediaminetetraacetic acid tetrasodium salt, by Merck Group (1 mentions)
Superoxide dismutase sod from bovine erythrocytes, by Merck Group (1 mentions)
Glutathione reductase from baker s yeast, by Merck Group (1 mentions)
Sodium azide, by Merck Group (1 mentions)
Ethyl acetate, by Thermo Fisher Scientific (1 mentions)
Glycine, by Merck Group (1 mentions)
Chlorhexidine, by Merck Group (1 mentions)
4 protocols using 2 thiobarbituric acid
1
Antioxidant Capacity Evaluation Protocol
2
Glutathione Reductase Assay Protocol
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Glutathione reductase (GR) from baker's yeast (S. cerevisiae) (100-300 U mg -1 protein), superoxide dismutase (SOD) from bovine erythrocytes (3000 U mg -1 protein), xanthine oxidase from bovine milk (0.4-1.0 U mg -1 protein), ethylenediaminetetraacetic acid tetrasodium salt, nitrotetrazolium blue chloride (NBT), xanthine, and potassium cyanide were purchased from Sigma Aldrich (Germany). A stabilised 3 % solution of hydrogen peroxide was purchased from Fluka (Germany), β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt ( N A D P H ) f r o m S e r v a ( G e r m a n y ) , a n d ethylenediaminetetraacetic acid disodium salt (EDTA-2Na) from Pharmacia Biotech (Sweden). Acetonitrile and dimethyl sulfoxide (DMSO) were purchased from J.T. Baker (Italy), yeast extract (YES) from Biolife (Italy), while aflatoxin standard mix (AFB1, AFG1, AFB2, and AFG2) from Biopure (Austria). Sodium azide and hydrochloric acid were purchased from Merck (Germany), trichloroacetic acid from Kemika (Croatia), ethanol absolute from Panreac (Spain), and butylated hydroxytoluene and 2-thiobarbituric acid were purchased from Acros Organics (USA). All other chemicals were of p.a. quality and purchased from commercial suppliers.
3
Multidisciplinary Reagent Preparation for Research
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For the experiments, we have used various reagents, such as MacConkey broth, Luria bertani broth, Guanidine hydrochloride, Potassium Phosphate dibasic, Potassium phosphate monobasic, Sodium dodecyl sulfate, sodium hydroxide, Nitroblue tetrazolium (NBT), potassium hydroxide, 2,4-dinitrophenylhydrazine (DNPH), 2-Thiobarbituric acid (TBA), and Dimethyl sulfoxide, that were purchased from Himedia Laboratories Ltd., India. Chlorhexidine was purchased from Sigma Aldrich, Hydrochloric acid, sodium chloride and glycine were from Merck, India. Glacial acetic acid and glycerol from Qualigens, India. Ethyl acetate purchased from Fisher scientific.2-Thiobarbituric acid (0.8%) was prepared in 1M NaOH,2,4-dinitrophenylhydrazine (10mM) was prepared in 2N HCl. For PI staining, 100mM Tris (pH 7.4), 150mM NaCl, 1mM CaCl2, 0.5mM, MgCl2, and 0.1% Triton-X were prepared, 2.5% glutaraldehyde, and ethanol.
4
Microencapsulation of Pomegranate Peel Polyphenols
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Polyphenol‐containing extract (PPP) isolated from Georgia‐grown pomegranate peels were microencapsulated by following the method previously reported by our group (Yang et al., 2022 (link)). In short, PPP was homogenized with a mixture of maltodextrin: pomegranate peel pectin (ratio 3:1, w/w) to create PPP suspensions (core: wall materials = 1:5, w/w). Then, the PPP suspensions were spray‐dried to produce microencapsulated MPP powders. A commercial grape seed extract (GSE) (Grape seed extract, Zazzee, Montebello, NY, USA) was purchased from a local store in Griffin, GA. Peanut oil, white wine vinegar, salt, red pepper flakes, garlic powder, basil leaves, and oregano were obtained from a local supermarket in Griffin, GA, USA. Iodine monochloride Wijs solution, chloroform, potassium iodide, sodium thiosulfate, starch indicator, glacial acetic acid, iso‐octane, 1, 1, 3, 3‐tetraethoxtpropane, trichloroacetic acid, and 2‐thiobarbituric acid were obtained from Fisher Scientific (Fair Lawn, NJ, USA).
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