A Hunter Lab colorimeter was used to measure the fish color difference values (Lab value) of hairtail lateral lines in the front, middle, and back positions (Colorquest II, Hunter Associates Laboratory Inc., Reston, VA, USA). The hairtail were dissected and skinned, and the muscles (3 × 3 × 2 cm) above the fish’s anterior, middle, and posterior lateral lines were cut to measure the color difference (Lab value), with the average value of the three parts used as the result.
Color quest 2
Manufactured by HunterLab
Sourced in United States
The Color Quest II is a spectrophotometric instrument designed for color measurement and analysis. It provides accurate and reliable color data, allowing users to assess the color properties of various materials and surfaces.
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Lab products found in correlation
4 protocols using color quest 2
1
Hairtail Lateral Line Color Analysis
2
Physicochemical Characterization of Samples
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The luminosity (L*) of samples was determined by means of colorimeter (Hunter-Lab, Color Quest II, Reston, USA); apparent viscosity (AV) at 25°C was measured in a viscometer (Brookfield, DV-II +, Middleboro, USA) at speed of 50 cP, spindle 2 (LV2); titratable acidity (TA) was obtained by titration according to AOAC method No. 942.15 (2012) , expressed as citric acid concentration; and the TSS content was read in digital refractometer (Reichert, R 2 mini, New York, USA), expressed in ºBrix, according to AOAC method No. 932.12 (2012) . The TSS / TA ratio was also calculated. All analyses were performed in triplicate.
3
Color Stability of Botanical Extract
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Measures of color were taken in JPT at room temperature using a spectrophotometer (Color Quest II e Hunter Lab, Reston, USA). CIELAB* system (D65, 10 observer, transmittance) was used and parameters a*, b*, and L* were registered. The extract was divided into two amber bottles, one was stored at refrigerated conditions (at 4e10 C) and the other was kept at room temperature (22e23 C). Color measurements of each bottle were taken and compared to D0, considered standard, by three days (72 h). Measurements were taken after the bottles reached room temperature. Color difference was calculated (DE) according to Equation (4):
Where L*, a*, and b* are color parameters; day 0 ¼ standard; day X ¼ 24 h, 48 or 72 h after storage.
In addition, it was carried out the following assays: total phenolic, monomeric anthocyanins and FRAP, as described in the Sections 2.2.1.1 and 2.2.1.2, in order to evaluate also the stability of bioactive compounds.
Where L*, a*, and b* are color parameters; day 0 ¼ standard; day X ¼ 24 h, 48 or 72 h after storage.
In addition, it was carried out the following assays: total phenolic, monomeric anthocyanins and FRAP, as described in the Sections 2.2.1.1 and 2.2.1.2, in order to evaluate also the stability of bioactive compounds.
4
Colorimetric Analysis of Bittersweet Chocolates
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The colour of the bittersweet chocolates was determined according to the CIELab system. The parameters L * (brightness), a * (redness) and b * (yellowness) were measured in a spectrophotometer (Color Quest II model, Hunter Associates Laboratory, Reston, VA, USA), adjusted with the following parameters: 10°a ngle, illuminant D65 and calibration mode RSIN (Minolta, 1994) .
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