Sodium deoxycholate (SDC), tris(2-carboxyethyl)phosphine (TCEP), iodoacetamide (IAA), formic acid (FA), acetonitrile and ammonium bicarbonate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pierce-BCA protein assay kit was purchased from Thermo-Fisher (Waltham, Massachusetts, USA). Proteomics sequencing grade modified trypsin was purchased from Promega (Madison, WI, USA).
Proteomics sequencing grade modified trypsin
Manufactured by Promega
Sourced in United States
Proteomics sequencing grade modified trypsin is a laboratory reagent used for the digestion of proteins prior to mass spectrometric analysis. It is an enzyme that specifically cleaves peptide bonds on the C-terminal side of lysine and arginine amino acid residues, generating peptide fragments suitable for downstream proteomic applications.
Automatically generated - may contain errors
Lab products found in correlation
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Sodium deoxycholate, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Iodoacetamide, by Merck Group (1 mentions)
Ammonium bicarbonate, by Merck Group (1 mentions)
Hplc grade water, by Thermo Fisher Scientific (1 mentions)
2 protocols using proteomics sequencing grade modified trypsin
1
Protein Quantification and Digestion
2
Trypsin-based Proteomic Sample Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise indicated. Proteomics sequencing-grade modified trypsin was from Promega (Madison, WI, USA). Water (HPLC grade) and acetonitrile (ACN, HPLC grade) were purchased from Thermo Scientific Inc. (Bremen, Germany).
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